The invention relates to a biological essence and polypeptide composition as well as technical preparation and application of lipidosome nanocrystallization of the biological essence and polypeptide composition. The composition is prepared from oligopeptide-1, tripeptide-1, hexapeptide-9, palmitoyl pentapeptide-4, palmitoyl tripeptide-5, palmitoyl tetrapeptide-7, algal polysaccharides, a moutan bark extract, a radix scutellariae extract, arbutin, a stephania tetrandra extract, a radix gentianae extract, a citrus extract, a chamomilla recutita flower extract, a polygonum cuspidatum extract, a centella asiatica extract and water. According to the invention, human skin tests show that facial skin problems, such as darkness, stains, wrinkles and the like, can be remarkably improved, and the compound liposome of the plant essence and the polypeptide composition has the effect of remarkably improving the skin problems.