The invention discloses a two-dimensional catalytic material and preparation and application thereof. The two-dimensional catalytic material is characterized in that a transition metal foil with the micron-order thickness serves as a base material, a two-dimensional matrix carrier with light weight, flexibility, high specific surface area and roughness is prepared through pretreatment, anodic oxidation, acid etching regulation and control, hole breaking and thermal stabilization treatment, and on the basis of the carrier, the two-dimensional ozone catalytic material is prepared by taking metal salt and/or organic matter as a precursor and adopting vacuum impregnation, in-situ deposition and thermal reduction technologies to load active metal and/or non-metal active components in situ. The two-dimensional material has the advantages of high specific surface area, high roughness, high mechanical strength, light weight, flexibility and the like; when applied to water treatment, the catalytic material has the advantages of rapid interface mass transfer, excellent catalytic performance and the like; and meanwhile, the preparation process of the two-dimensional catalytic material has the characteristics of simple method, easiness in adjustment of process parameters, easiness in large-scale production, low preparation cost and the like.