The invention discloses a preparation method of minty sliced dried beef. The minty sliced dried beef is matched with mint syrup and is fragrant, crispy and very delicious after being eaten. In the minty sliced dried beef, highland barley powder is added and comprises protein, fat, starch and vitamin B, so that the minty sliced dried beef has the effects of tonifying the spleen, nourishing the stomach, tonifying qi, preventing diarrhea, strengthening sinew, enhancing energy, removing humidification and sweating. According to the preparation method, a staged baking process is adopted, so that the content of water in the sliced dried beef and the water activity of the sliced dried beef can be fast reduced, sterilization and cooking functions can be realized, and further the minty sliced dried beef has roasting fragrance. Microorganisms are used for fermenting raw beef so as to obtain beef products which keep the original features of conventional beef and also have the obvious improvement on the aspects of quality, color, luster, taste, flavor and shelf life. In addition, a plurality of Chinese herbal medicines are added in the machining process, so that the minty sliced dried beef has the effects of improving eyesight, strengthening the brain, eliminating stagnant blood, stopping bleeding, removing swelling, relieving pain, clearing heat and inducing diuresis.