The invention relates to a negative ion high-pressure hose and a preparation method thereof, and the preparation method is characterized by comprising the following steps: mixing 100 parts by weight of crude rubber, 2-4 parts by weight of zinc oxide, 0.5-1.5 parts by weight of stearic acid, 55-65 parts by weight of carbon black N770, 55-60 parts by weight of stearic pot clay, 18-22 parts by weight of plasticizer POP, 4-6 parts by weight of tackifying resin, 1-2 parts by weight of accelerator CZ, 0.2-0.4 part by weight of accelerator D, 1-2 parts by weight of sulphur, 0.8-1.2 part by weight of chalked cross-linking agent TAIC, 0.3-0.6 part by weight of antiager BLE and 1-3 parts by weight of negative ion powder, mixedly compressing, extruding with skeletons, moulding, and vulcanizing to obtain the product. The obtained product has the advantages and effects of possessing anti-static function, preventing feed liquor from corrosion, removing odor from odorous feed liquor, purifying air and resisting electromagnetic radiation, and having high temperature resistance and high pressure resistance.