The invention relates to micro-emulsified biological diesel oil and a method for preparing the same. The micro-emulsified biological diesel oil is characterized by comprising the following substances in percentage by weight: wherein 50 to 60 percent of mineral diesel oil, 25 to 30 percent of animal grease, 10 to 15 percent of methanol, 0.5 to 3.0 percent of naphthenic acid, 0.5 to 3.0 percent of OP emulsifier, 0.5 to 2.0 percent of sodium dodecyl sulfonate and 0.5 to 2.0 percent of trolamine. The method for preparing the micro-emulsified biological diesel oil comprises the following steps: the animal grease, the methanol, the OP emulsifier, the sodium dodecyl sulfonate and the trolamine are evenly mixed according to weight percentage; the mixed solution is slowly poured into the mineral diesel oil under the stirring condition; simultaneously, according to the requirement of the weight percentage, the naphthenic acid is added and fully stirred and mixed, thereby preparing the micro-emulsified biological diesel oil. The micro-emulsified biological diesel oil has the advantages of high combustion efficiency, small environmental pollution, simple preparation process, convenient use and good stability and can be used as commercial oil for mass popularization and use.