32 results about "Copper ion sequestering" patented technology

The invention discloses a method for preparing peanut antioxidant peptide by fermentation, and the method comprises the following steps: adding distilled water to low-denatured degreased peanut protein powder, ultrasonically dispersing and dissolving, adjusting the pH value, sterilizing and cooling; inoculating with strain seed culture liquid, and fermenting and culturing; and carrying out ultrasonic-assisted enzymolysis, inactivating enzyme, centrifuging, and freeze-drying supernatant to obtain the peanut antioxidant peptide. The peanut antioxidant peptide prepared by the method disclosed by the invention has the antioxidant activities of scavenging hydroxyl free radical, superoxide anion free radical and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical, inhibiting hydroxyl-free-radical-mediated deoxyribose injury, inhibiting peroxidation of lipidosome, resisting oxidation of linolic acid system, resisting grease peroxidation capability and the like, and has iron reduction capability, molybdenum reduction capability, iron ion chelating capability and copper ion chelating capability; and the method is simple to operate, has mild reaction conditions and is suitable for industrial production.

The invention discloses a preparation method of a copper ion chelated nano particle biological coating. The preparation method comprises the following steps: at first, chelating PLL and copper ions to obtain a PLL / copper ion compound; utilizing the characteristic that PLL and chondroitin sulfate can carry out electrostatic interaction to mix PLL / copper ion compound with chondroitin sulfate so as to form copper ion chelated nano ions; then preparing a DM coating on the surface of 316L stainless steel, utilizing the characteristic that DM and primary amino group can carry out Schiff base reactions to fix nano particles containing amino groups onto the surface of a sample so as to establish a multifunctional surface that can prevent coagulation and proliferation and promote endothelial regeneration. By establishing a nano particle coating, which has multiple biological functions, on the surface f 316L stainless steel, the anti-coagulation and anti-proliferation functions of stainless steel are prominently enhanced, and at the same time, the performance of repairing endothelial injury is strengthened.

The invention discloses a glyphosate detecting method. Green fluorescence of coumarin is quenched by the electron transfer effect on the basis of copper ions; carboxyl and phosphate groups on glyphosate and the copper ions can be chelated to form a compound, so that the fluorescence of coumarin is recovered. The detecting method has the advantages that in the glyphosate detecting process, the operation is simple; the detection specificity is high; the detection sensitivity is high; the response speed is high; the result is sensitive and reliable; the cost is low; the real-time online fast andspecific detection can be realized; the method can be used for simple and fast detection on glyphosate pesticide residues in tap water and rice. Good application prospects and potential application values are realized in the fields of detection and analysis.

The invention relates to the technical field of preparation of conductive metal materials, in particular to a preparation method of a high-strength high-conductivity copper-based composite material. According to the preparation method of the high-strength high-conductivity copper-based composite material, firstly, porous structures are generated on the surfaces of rice hulls under the effect of biogas slurry microorganisms, then porous silicon carbide whiskers are formed through thermal transition under the catalytic action of iron powder, then polydopamine thin films adhere to the surfaces ofthe porous silicon carbide whiskers and micropores, and copper ions are fixed to the surfaces of the silicon carbide whiskers and internal pore spaces in a chelated mode through the polydopamine thinfilms; the polydopamine thin films are decomposed under the conditions of hydrogen and the high temperature firstly so that the copper ions can be exposed again, then the copper ions are reduced intopure copper through hydrogen, thus the compatibility between the silicon carbide whiskers and copper substrates is improved, and through vortex-shaped mutual jetting collision, internal titanium andsilicon carbide generate a chemical reaction to generate novel ceramic-reinforced TiC, Ti3SiC2 and TiSi2 phases so that the finally-prepared copper-based composite material can have the strength and electrical conductivity at the same time; and the preparation method has the wide application prospects.

A treatment method of cupriferous sludge comprises the following steps: acid dissolution: the cupriferous sludge is dissolved in sulfuric acid solution to form a first mixture comprising cupriferous acidified sludge; copper refining: a ferric chloride solution is added into the first mixture to react with the cupriferous acidified sludge in the first mixture to form a second mixture; copper extraction: the second mixture and a resin material are mixed to react to form a third mixture comprising a cupric ion-containing chelate; copper regeneration: a sulfuric acid solution is added into the third mixture so that the copper ion chelate in the third mixture reacts with sulfuric acid to form a cupric sulfate solution; and electrolysis: the cupric sulfate solution is electrolyzed to form a cupriferous material.

The invention relates to a nutritional type copper fungicide and a preparation method. Microelements such as boron, zinc, manganese, molybdenum and the like are added to a copper ammonia complex, ethylenediamine having a bactericidal function and an efficient copper ion chelating agent is added, meanwhile, citric acid capable of promoting the tricarboxylic acid cycle is added to be used as a complexing agent, EDTA (ethylene diamine tetraacetic acid) and the like are added to be used as chelating agents, and a liquid compound which has a copper protective bactericidal function and a systemic bactericidal function and is added with the microelements to promote growth of crops is formed. According to the deep bluish violet compound solution, extremely few water insoluble matters exist, the concentration of free copper ions is very low, the antibacterial spectrum is wide, effects of controlling blight, powdery mildew, early blight and the like (endogenous and soil-borne plant diseases) of the crops are good, phytotoxicity does not occur easily, and growth and yield increase of the crops can also be promoted. The production process is simple and easy to implement, raw materials are easy to obtain, the production cost is lower, and popularization is facilitated.

The invention relates to a preparation method of copper nanowires and in particular relates to a controllable preparation method of high-yield monocrystal copper nanowires in a liquid-phase reduction manner. The controllable preparation method comprises the steps: firstly, mixing a divalent metal copper salt solution and ethylenediamine, and heating in a water bath to form a single and stable copper ion chelate compound; mixing a strong base solution with hydrazine hydrate, and preparing a reducing agent in the water bath at the same temperature; then transferring the two mixture solutions into a reaction vessel, fully stirring to reach a uniform state, covering well, and placing in the water bath at the same temperature for heating reaction to prepare the copper nanowires; and fishing out flaky copper nanowires floating on the upper layer of the solution, washing the flaky copper nanowires with deionized water and absolute ethyl alcohol in sequence three times, and drying at room temperature under an Ar gas protection condition to prepare the copper nanowires. The controllable preparation method provided by the invention has the advantages of simple process, low cost and high yield, and the prepared copper nanowires are of monocrystal structures, and have relatively smooth surfaces, uniform radial thickness, and controllable and more uniform length and diameter.

The invention provides a synthesis method for preparing a heavy metal copper artificial antigen based on a novel difunctional chelating agent NOTA (2-S-(4-amino-benzene)-1,4,7-triazacyclononane-1,4,7-triacetic acid). According to the preparation method, NOTA is taken as the chelating agent, a copper ion chelating agent compound is coupled with BSA (bovine serum albumin) as carrier protein or chicken OVA (ovalbumin), and the artificial antigen is prepared. According to the method, the step of sodium borohydride reduction is added on the original traditional basis, so that the coupling efficiency is improved. The invention further provides an application of NOTA to preparation of the heavy metal copper artificial antigen.

The invention discloses a method for measuring the influence of chelant additive quantity on the chelating quantity of copper ions. The method comprises the steps of: preparing a heavy metal chelant for later use; preparing a copper ion solution; respectively adding chelant solutions in different quantities to the copper ion solution; taking out the supernatant liquors respectively to measure the absorbance after agitation; solving the quantity of chelate Cu<2+>; and obtaining the influence on the removal rate according to the quantity of chelate Cu<2+>. The method disclosed by the invention can be used for rapidly measuring the influence of the chelant additive quantity on the chelating quantity of the copper ions, has high measurement precision and simple measurement steps, is beneficial to reduction of the measurement cost and provides a good data base for use of the chelant.

The invention relates to the field of treatment of smoking-related respiratory system diseases, in particular to an application of oligopeptide and copper ion chelate thereof in prevention and treatment of lung smoking-related respiratory system diseases. The invention discloses an application of the oligopeptide and the copper ion chelate thereof in preparation of a medicine for preventing and / ortreating the smoking-related respiratory system diseases. An amino acid sequence of the oligopeptide is Gly-His-Lys. Experiments prove that the GHK oligopeptide and the copper ion chelate thereof caninhibit the occurrence of smoking-related diseases of mice by antagonizing oxidative stress reaction and inflammatory reaction, and inhibit the occurrence of collagen deposition and airway remodelingaround the airway by antagonizing epithelial mesenchymal transition, thereby inhibiting the occurrence and development of the smoking-related respiratory diseases.

The invention relates to the field of silicosis treatment, in particular to an application of oligopeptide and a copper ion chelate thereof in prevention and treatment of silicosis. The invention discloses an application of the oligopeptide and the copper ion chelate thereof in preparation of a medicine for preventing and / or treating the silicosis. An amino acid sequence of the oligopeptide is Gly-His-Lys. Experiments prove that the GHK oligopeptide and the copper ion chelate thereof can inhibit the formation of silicon nodules and collagen deposition through antagonizing oxidative stress reaction and epithelial mesenchymal transition, and inhibit lung inflammatory reaction, thereby inhibiting the occurrence and development of silicon-induced pulmonary fibrosis, namely the silicosis.

The invention discloses instant dried tilapia and a processing method thereof, and relates to the technical field of food processing. The method comprises the following steps: pretreating tilapia to obtain diced tilapia and tilapia bones; diced fish is pickled for a certain time, then the diced fish is fried at a high temperature, the crispy taste of the dried fish is kept, the copper-bone meal microspheres are fixed on the diced fish by utilizing an ultrasonic-assisted high-voltage pulsed electric field, then the diced fish is pickled with brine, and then the pickled diced fish is fully mixed with seasonings to prepare the dried fish with different tastes so as to be suitable for different people. Wherein the copper-bone powder microspheres are obtained by puffing fish bones by infrared freezing-variable temperature and pressure difference, and calcium ions and amino acids are dissolved out; after being subjected to superfine grinding, the dried tilapia mossambica is chelated with copper ions, and calcium and copper can be supplemented at the same time when the dried tilapia mossambica is eaten; then, the copper-bone powder microspheres are prepared through ultrasonic-assisted spray cooling, and absorption of the human body to calcium and copper is improved. The instant dried tilapia processed by the processing method disclosed by the invention is fragrant and crispy, and can achieve the effect of supplementing nutrition.

The invention discloses an enzyme linked immunosorbent assay kit used for detecting the content of heavy metal copper ions in a sample. The kit includes a coat antigen coated enzyme label plate, an enzyme marker, a copper ion chelate specific monoclonal antibody or polyclonal antibody, a copper ion chelate standard solution, a substrate color development solution, a terminating solution, a lotion, and a diluted chelating agent EDTA. The enzyme linked immunosorbent assay kit used for copper ion detection has the advantages of: (1) strong specificity, high sensitivity, a lowest detection limit of 0.42 microgram / L, and a detection range of 2.83-456.04 microgram / L; (2) simplicity, rapidity, and strong timeliness; (3) vivid, visual and accurate result display; and (4) low cost, wide application range, convenient popularization, on-site supervision and suitability for screening a large number of samples.

The invention discloses a method for preparing peanut antioxidant peptide by fermentation, and the method comprises the following steps: adding distilled water to low-denatured degreased peanut protein powder, ultrasonically dispersing and dissolving, adjusting the pH value, sterilizing and cooling; inoculating with strain seed culture liquid, and fermenting and culturing; and carrying out ultrasonic-assisted enzymolysis, inactivating enzyme, centrifuging, and freeze-drying supernatant to obtain the peanut antioxidant peptide. The peanut antioxidant peptide prepared by the method disclosed by the invention has the antioxidant activities of scavenging hydroxyl free radical, superoxide anion free radical and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical, inhibiting hydroxyl-free-radical-mediated deoxyribose injury, inhibiting peroxidation of lipidosome, resisting oxidation of linolic acid system, resisting grease peroxidation capability and the like, and has iron reduction capability, molybdenum reduction capability, iron ion chelating capability and copper ion chelating capability; and the method is simple to operate, has mild reaction conditions and is suitable for industrial production.

The invention relates to the field of treatment of acute and chronic bronchial asthma, in particular to application of a tripeptide-based compound and a copper ion chelate thereof in treatment of bronchial asthma. Experiments prove that the tripeptide compound GHK (glycyl-L-histidine-L-lysine) and the copper ion chelate of the tripeptide compound GHK can play a protective role in acute and chronic bronchial asthma by resisting inflammation, regulating immunity, resisting oxidation and reversing an epithelial cell mesenchymal phenomenon.

The invention discloses a preparation method of a copper ion chelated nano particle biological coating. The preparation method comprises the following steps: at first, chelating PLL and copper ions to obtain a PLL / copper ion compound; utilizing the characteristic that PLL and chondroitin sulfate can carry out electrostatic interaction to mix PLL / copper ion compound with chondroitin sulfate so as to form copper ion chelated nano ions; then preparing a DM coating on the surface of 316L stainless steel, utilizing the characteristic that DM and primary amino group can carry out Schiff base reactions to fix nano particles containing amino groups onto the surface of a sample so as to establish a multifunctional surface that can prevent coagulation and proliferation and promote endothelial regeneration. By establishing a nano particle coating, which has multiple biological functions, on the surface f 316L stainless steel, the anti-coagulation and anti-proliferation functions of stainless steel are prominently enhanced, and at the same time, the performance of repairing endothelial injury is strengthened.

The invention discloses a fluorine-contained organic metal porous material. A preparation method of the fluorine-contained organic metal porous material comprises the following steps: adding bisphenolAF, tripolycyanamide and copper sulfate pentahydrate into dimethylbenzene, and heating while stirring for reaction; in the reaction, removing water produced during the reaction by using a water separator, and producing a black precipitate during the reaction; filtering the obtained black precipitate, washing with hot water and then drying to obtain a photocatalyst material. Due to existence of fluorine, the material has improved stability, is not easily oxidized, is stable under light irradiation and can be reused; through chelating of amino groups and copper ions, the stability of an organometallic structure is improved; the porous material has a large surface area, so that catalytic sites are increased, and a refractory organic pollutant phenol can be efficiently degraded, light absorption can be effectively improved and the photodegradation speed can be increased.

The invention provides a preparation method of copper ion chelating peptide as a green feed additive. The preparation method comprises steps as follows: (1), preparation of dry anchovy polypeptide powder: frozen anchovy is unfrozen and then is cleaned with clear water and uniformly chopped into meat paste, the meat paste is dispersed in distilled water, fish pulp is formed, PH value of the fish pulp is regulated to 6.5-7.5, exogenous enzyme is added for enzymolysis, enzymatic hydrolysate is formed, enzyme in the enzymatic hydrolysate is inactivated, then the enzymatic hydrolysate is subjectedto centrifugal treatment, a supernatant is taken, concentrated and dried, and the dry polypeptide powder is obtained; (2), preparation of copper ion chelating peptide: a peptide liquid is prepared from the dry polypeptide powder, PH value of the peptide liquid is regulated to 6.8-7.5, a soluble copper solution is added to the peptide liquid for a chelation reaction, centrifugal treatment is performed after the chelation reaction, precipitate is concentrated and dried, and the copper ion chelating peptide is obtained. According to the preparation method, the ratio of protein peptide with molecular weight distribution being 500 Da or lower in the peptide liquid is higher than 85%, therefore, the prepared copper ion chelating peptide is absorbed better by animals, and copper absorption rate is effectively increased.

The invention belongs to the technical field of biology, and particularly relates to a targeted drug-loaded micelle loaded with a copper ion chelating agent. According to the targeted drug-loaded micelle, the copper ion chelating agent and the chemotherapeutic drug are jointly loaded in the same nano-drug system and are delivered to a tumor area in a targeted manner, and the purpose of inhibiting tumor growth is achieved through the cytotoxic effect of chelating copper ions in the tumor area and the chemotherapeutic drug; meanwhile, the copper ion chelating agent applied in the invention can emit near-infrared fluorescence after chelating copper ions. And finally, the aim of integrated diagnosis and treatment of tumors is fulfilled.

The invention provides a method for enriching low concentration copper in water through a carrageenan chelating technology. The method comprises the following steps: dissolving carrageenan powder, mixing obtained carrageenan solution with a standard copper ion solution, chelating carrageenan and copper ions to obtain a chelate, dialyzing the chelate, adding hydrochloric acid to break the chelate structure, detecting obtained copper ion enriched liquid by using an atomic spectrophotometer, and calculating according to a standard curve of the standard copper ion solution in order to obtain the copper content. The method improves the detection precision of copper in a water sample with low concentration of copper ions, is simple, and is easy to implement.

The invention relates to a nutritional copper bactericide and a preparation method thereof. Trace elements such as boron, zinc, manganese and molybdenum are added to the copper ammonium complex, ethylenediamine having a bactericidal function and a high-efficiency copper ion chelating agent is added, and at the same time The citric acid that promotes the tricarboxylic acid cycle is used as a complexing agent and EDTA is added as a chelating agent to form a liquid compound that not only has copper protective and systemic bactericidal functions, but also supplements trace elements to promote crop growth. In this dark blue-purple compound solution, there are very few water-insoluble substances, the concentration of free copper ions is very low, and the antibacterial spectrum is wide. It has the effect of preventing and controlling crop wilt, powdery mildew, early blight, etc. Good, and not prone to phytotoxicity, and can promote crop growth and yield. The production process is simple and easy, the raw materials are easy to obtain, the production cost is low, and the promotion is convenient.

The invention relates to the technical field of preparation of conductive metal materials, in particular to a preparation method of a high-strength high-conductivity copper-based composite material. According to the preparation method of the high-strength high-conductivity copper-based composite material, firstly, porous structures are generated on the surfaces of rice hulls under the effect of biogas slurry microorganisms, then porous silicon carbide whiskers are formed through thermal transition under the catalytic action of iron powder, then polydopamine thin films adhere to the surfaces ofthe porous silicon carbide whiskers and micropores, and copper ions are fixed to the surfaces of the silicon carbide whiskers and internal pore spaces in a chelated mode through the polydopamine thinfilms; the polydopamine thin films are decomposed under the conditions of hydrogen and the high temperature firstly so that the copper ions can be exposed again, then the copper ions are reduced intopure copper through hydrogen, thus the compatibility between the silicon carbide whiskers and copper substrates is improved, and through vortex-shaped mutual jetting collision, internal titanium andsilicon carbide generate a chemical reaction to generate novel ceramic-reinforced TiC, Ti3SiC2 and TiSi2 phases so that the finally-prepared copper-based composite material can have the strength and electrical conductivity at the same time; and the preparation method has the wide application prospects.

The invention discloses a glyphosate detecting method. Green fluorescence of coumarin is quenched by the electron transfer effect on the basis of copper ions; carboxyl and phosphate groups on glyphosate and the copper ions can be chelated to form a compound, so that the fluorescence of coumarin is recovered. The detecting method has the advantages that in the glyphosate detecting process, the operation is simple; the detection specificity is high; the detection sensitivity is high; the response speed is high; the result is sensitive and reliable; the cost is low; the real-time online fast andspecific detection can be realized; the method can be used for simple and fast detection on glyphosate pesticide residues in tap water and rice. Good application prospects and potential application values are realized in the fields of detection and analysis.

The invention discloses copper ion chelated carboxyl functionalized magnetic bentonite as well as a preparation method and application thereof, and belongs to the technical field of magnetic materialpreparation and proteomics. The preparation method comprises the following steps: firstly, synthesizing carboxyl functionalized magnetic bentonite in one step by adopting solvothermal method; then, adding the carboxyl functionalized magnetic bentonite into a copper sulfate solution, and mechanically stirring to prepare a reaction solution containing copper ion chelated carboxyl functionalized magnetic bentonite; and finally, separating the solid polymer from the reaction solution through an external magnetic field, and washing and drying the separated solid polymer to obtain the copper ion chelated carboxyl functionalized magnetic bentonite. The copper ion chelated carboxyl functionalized magnetic bentonite prepared by the invention is good in structural stability, strong in magnetic response, strong in selective adsorption capacity on bovine hemoglobin, large in adsorption capacity, high in recovery rate, low in material cost and simple to prepare.

The invention discloses a fluorine-containing organic metal porous material. Its preparation method includes: adding bisphenol AF, melamine and copper sulfate pentahydrate into xylene, heating and stirring for reaction; using a water separator to remove the water generated by the reaction during the reaction. , the reaction generates a black precipitate; filter the obtained black precipitate, wash with hot water and dry to obtain the photocatalyst material. The fluorine content of the present invention increases the material stability, is not easily oxidized, is stable under light, and can be reused. The amino group chelates with copper ions to increase the stability of the organic metal structure. The porous material has a large surface area and increases catalytic sites. It can efficiently degrade the refractory organic pollutant phenol, effectively improve light absorption and accelerate the photodegradation rate.

The invention discloses a selective copper ion chelating agent, its preparation method and its application in colorectal cancer. The chelating agent is N-(2-(pyridin-3-yl)thieno[3,2-c ]pyridin-4-yl)acetamide. Cu of the present invention + / Cu 2+ The chelating agent JYFY‑001 has a novel structure, and can inhibit the proliferation of colorectal cancer SW620 and HCT116 cells by specifically chelating copper ions enriched in colorectal cancer cells; it has shown a good ability to inhibit HCT116 microspheres; Lower toxicity to normal cells. The invention provides a compound capable of inhibiting the proliferation of colorectal cancer cells, microspheres of colorectal cancer cells and lower normal cytotoxicity. important meaning.

The invention discloses a method for regenerating a copper rod from a waste copper wire and a regenerated copper rod. The method comprises: (1) peeling the waste copper wire and making waste copper particles; (2) using a polishing composition to make The waste copper particles are polished, filtered and dried; the polishing composition includes a first polishing agent, a second polishing agent and water, and the components of the first polishing agent include a copper ion chelating agent, an organic acid, a brightening agent and a dispersing agent. The second polishing agent is sulfuric acid; (3) the treated waste copper particles are heated to the first temperature, kept warm, and hot-pressed into copper billets, and then the copper billets are heated to the second temperature, kept warm, and hot-extruded into copper rods; the first The temperature is 800-850°C, and the second temperature is increased by 50-100°C relative to the first temperature; and the regenerated copper rod made by the above method avoids problems such as low recovery rate, high cost, environmental pollution and the like of existing recycling methods , the low-oxygen copper rod was obtained in a green and environmentally friendly manner.