The invention provides a preparation method of 
aroma-increasing buffalo yogurt and belongs to the technical field of 
food processing. According to the preparation method, raw buffalo milk serves as 
raw milk and is subjected to part skimming treatment so that partly 
skimmed milk can be obtained, then a specially developed buffalo 
milk fat natural 
aroma-increasing base material is added, a proper quantity of strains are added for 
fermentation after homogenizing, sterilization and cooling are carried out, and thus the 
aroma-increasing buffalo yogurt is obtained. In the operation process of the preparation method, the buffalo 
milk fat natural aroma-increasing base material is prepared by using the raw buffalo milk or 
single cream obtained through skimming or part skimming of the raw buffalo milk as an enzymolysis substrate and carrying out enzymolysis with 
flavor proteinase and fat proteinase in sequence. The obtained aroma-increasing base material is stable in quality and rich in 
flavor and is added for preparation of yogurt, so that comprehensive utilization of by-product fat is achieved. Meanwhile, the base material has abundant small molecular 
peptide, 
amino acid and 
fatty acid, pH is lower than that of the 
raw milk, buffalo milk 
fermentation and yogurt coagulation can be promoted, the prepared buffalo yogurt product is rich in lactoprotein-derived 
flavor and 
milk fat-derived flavor, and the 
fat content is low.