The invention relates to an anti-wrinkle skin makeup preparation combined by natural plant essence and active mineral substances. The anti-wrinkle skin makeup preparation comprises tourmaline and argenon which serve as the active mineral substances and ginseng, glossy ganoderma, white poria, prepared tuber of multiflower knotweed, pearl powder, baical skullcap root, lightyellow sophora root, root of red-rooted salvia and peach seed which serve as natural plants. A preparation method comprises the following steps of: washing the prepared tuber of multiflower knotweed, the baical skullcap root and the like, immersing, performing reflux extraction under reduced pressure, concentrating, performing alcohol precipitation, filtering, and extracting supernate; washing the ginseng, the glossy ganoderma and the white poria, performing reflux by ethanol, percolating, and extracting a concentrated solution; preparing a pearl powder hydrolytic solution by using the pearl powder; grinding the tourmaline and the argenon into mineral powder; and mixing the supernate, the concentrated solution and the hydrolytic solution uniformly, filtering, extracting supernate, adding the supernate into the mineral powder, stirring and immersing to obtain the anti-wrinkle skin makeup preparation. The anti-wrinkle skin makeup preparation exerts the effects of moistening, antiallergy, repair, balancing, radical resistance, wrinkle improvement, anti-aging, the promotion of cytothesis and collagen synthesis; and the anti-wrinkle skin makeup preparation has an obvious curative effect, and is safe and reliable, a formula is scientific, a preparation method is unique, a treatment course is short, and quick response is ensured.