The invention belongs to the technical field of pipeline linings, and discloses an isobaric preparation process for a polytetrafluoroethylene lining of a pipeline. The process comprises the followingsteps of filling polytetrafluoroethylene powder between the inner wall of the pipeline and a rubber bag soft mold, sealing, putting into an autoclave, equally increasing the water pressure in a rubberbag and the autoclave to a specific high pressure, and expanding the rubber bag to compact polytetrafluoroethylene, so that forming the uniform and compact lining on the inner wall of a steel pipe. Compared with a traditional compression molding method, the isobaric molding process has the advantages that equipment and molds used are simple, operation is convenient, and the yield is high; the pressures inside and outside the pipeline are equal in the machining process, and the steel pipe is not damaged; and the lining is uniform and compact and low in shrinkage rate due to everywhere equal high pressure. The specific technological process comprises the steps of A, steel pipe inner surface treatment, B, rubber bag soft mold loading, C, polytetrafluoroethylene powder adding, D, sealing, E,water injection and pressurization in the autoclave, F, pressure relief and part taking, G, rubber bag soft mold removal, H, sintering and I, inner filler removal.