The invention relates to a bisamide type ultra-long-life room-temperature phosphorescent compound and a preparation method and application thereof. Four pure organic room-temperature phosphorescent molecules with ultra-long service life are prepared by one-step reaction of N acylation of carbazole and acyl chloride, and thus four compounds, namely, DCED, o-PBCM, m-PBCM and p-PBCM are obtained, andsingle crystals of three compounds DCED, o-PBCM and p-PBCM and floccules of m-PBCM after recrystallization are obtained by using a recrystallization method in an ethanol solution. Compared with the prior art, the four substances obtained by the preparation method have ultra-long service life and room-temperature phosphorescent properties, and particularly, the phosphorescent service life of the compound m-PBCM obtained by the preparation method at room temperature is up to 710.6 ms. When the compound m-PBCM is applied to the field of biology, an m-PBCM nanoparticle solution is injected to theright back of a nude mouse for afterglow imaging, and the signal-to-noise ratio of the m-PBCM nanoparticle solution reaches up to 428; after being injected into a forepaw of a nude mouse, the m-PBCMnanoparticle solution can also be used as an effective afterglow contrast agent to accurately illuminate axillary lymph nodes with excellent sensitivity and signal-to-noise ratio.