52 results about "Localised inflammation" patented technology

This invention relates to the field of medical information management technology, including an AI-based endometriosis management system. The system comprises a thickness dynamic monitoring module, an inflammation level assessment module, a lesion spread analysis module, a periodic lesion tracking module, and a disease progression analysis module. In this invention, AI is used to segment ultrasound image data, enabling dynamic monitoring of lesion areas. Lesion boundaries are extracted, and changes in local inflammatory marker concentrations are calculated, making the screening of abnormal inflammatory areas more accurate and enabling early identification of lesion development. The tracking of periodic lesions, combined with analysis of lesion contour changes and area increases / decreases, enhances the quantitative control of disease progression. The future development trend of lesions, combined with the calculation of periodic inflammatory pathways, makes the prediction of disease evolution more consistent with physiological changes. Multi-dimensional lesion analysis combined with AI intelligent calculation expands the diagnosis and treatment of endometriosis from static assessment to dynamic trend prediction, improving the adaptability of personalized treatment plans.

The present disclosure relates to a functionalized statistic chitosan of weight average molecular mass between 100 kDa and 1000 kDa and of formula (I):whereineach Rc is independently a moiety comprising a chelating agent,each Z is independently a linker which is a single bond or a hydrocarbon chain containing between 1 and 12 carbon atoms, said hydrocarbon chain is linear or branched and optionally contains one or more unsaturations and one or more heteroatoms,x is between 0.005 and 0.7, preferably between 0.05 and 0.7,y is between 0.01 and 0.7, preferably between 0.05 and 0.2,the ratio y / x being greater than or equal to 0.05, preferably greater than or equal to 0.15, andthe sum x+y being greater than or equal to 0.15, for use in the treatment of diseases characterized by local inflammation induced by and / or inducing a deregulation of metal homeostasis and increased oxidative stress, in a subject in need thereof.

According to the application of the hydrogel preparation loaded with the quercetin nanoparticles to preparation of a chronic difficult-to-heal wound surface treatment material, Que-coated HZ hydrogel is obtained through physical loading of QueNPs and crosslinking of modified hyaluronic acid and Zn < 2 + >, full-stage management of wound healing can be achieved, rapid healing and tissue regeneration of the wound surface are promoted through the synergistic effect of all the components, and the healing effect of the wound surface is improved. A comprehensive treatment strategy is provided for chronic wound surfaces difficult to heal, and the Que-coated HZ hydrogel promotes transformation of macrophages from an M1 phenotype to an M2 phenotype and shows a good anti-inflammatory effect. In the early stage, the hydrogel preferentially releases Zn < 2 + > to achieve the antibacterial effect and relieve local inflammation, in the later stage, QueNps gradually releases Que to reduce ROS generation, so that the anti-oxidation and anti-inflammatory effects are achieved, wound repair is accelerated, and meanwhile the released Que can resist mitochondrial damage and improve the mitochondrial function.

The invention discloses a traditional Chinese medicine tea drink for preventing and treating oral mucosa diseases and a preparation method of the traditional Chinese medicine tea drink. The traditional Chinese medicine tea drink is prepared from 10-15 parts of radix astragali, 8-12 parts of radix codonopsis, 6-10 parts of radix salviae miltiorrhizae, 5-9 parts of radix angelicae sinensis, 3-6 parts of fructus psoraleae, 6-9 parts of rhizoma polygonati, 6-9 parts of radix adenophorae, 6-10 parts of rhizoma atractylodis macrocephalae, 5-9 parts of radix ophiopogonis, 3-5 parts of radix glycyrrhizae, 4-6 parts of pericarpium citri reticulatae and 5-8 parts of herba taraxaci. Aiming at the core pathogenesis of low immunity, long-term deficiency, long-term deficiency and kidney-yang insufficiency of patients suffering from oral mucosa diseases, qi tonifying, blood activating and kidney-qi strengthening are taken as a core treatment method, the formula is scientific and reasonable, all the medicinal materials act synergistically, the overall immune function of the body is adjusted, T lymphocyte subpopulation imbalance and cell factor network disorder are improved, and the curative effect is good. The oral mucosa microcirculation is improved, local inflammation is relieved, the immunity of a patient is improved fundamentally, the recurrence rate of oral mucosa diseases is reduced, and the problems that an existing tea drink is not high in pertinence and limited in curative effect are solved. The tea drink is appropriate in taste, good in compliance, high in safety and suitable for people with various oral mucosa diseases and low immunity.

This invention relates to a hyaluronic acid composite hydrogel based on the supramolecular self-assembly of natural molecules and its preparation method, belonging to the field of gel formulation technology. The invention first involves the self-assembly of specific natural molecules or their derivatives possessing hyaluronidase inhibitory and anti-inflammatory activities into stable supramolecular self-assemblies through intermolecular non-covalent interactions. Subsequently, this supramolecular self-assembly is combined with hyaluronic acid through various interactions such as physical entanglement, hydrogen bonding, van der Waals forces, or appropriate chemical cross-linking to construct a composite hydrogel with a uniform internal structure and stable performance. This invention not only achieves long-term, controllable sustained release of natural active ingredients in the gel through supramolecular loading, continuously inhibiting hyaluronidase activity and local inflammatory responses, but also avoids the residual risks associated with high dosages of traditional chemical cross-linking agents, ultimately obtaining a high-quality product that meets the appearance and performance requirements of medical hyaluronic acid gels.

The utility model discloses a dental crown fixing abutment structure convenient to disassemble, which relates to the technical field of medical equipment and comprises an implant retention screw, an abutment fixing seat is arranged on the outer side of the top of the implant retention screw, an abutment is butted on the abutment fixing seat, and a locking screw is arranged in the abutment. According to the dental crown fixing abutment structure convenient to disassemble, the abutment and the dental crown can be assembled and disassembled through fixing and disassembling of the locking screws, and the situation that the abutment is repeatedly taken and worn, mechanical stimulation is caused to gingival tissue when the abutment is taken off and worn, and local inflammatory response is induced is effectively avoided; multiple times of confirmation of in-position precision in the repeated wearing and taking-off process of the abutment are avoided, and the reworking frequency is reduced.

The invention belongs to the technical field of biological medicines, and particularly discloses application of a CD44 function inhibitor in preparation of a medicine for preventing or relieving radiation liver injury (RILD). Radioactive liver injury is often caused by hepatocellular carcinoma radiotherapy, and specific targeting drugs are lacked clinically. It is revealed for the first time that whether CD44 molecules on the surface of Kuhu cells are highly expressed after radiation, and the CD44 molecules are key hubs for driving RILD; the invention also provides a CD44 function inhibitor which is a specific targeting drug aiming at RILD, and the action mechanism of the CD44 function inhibitor is to effectively block polarization of radiation-induced Kuptake cells to pro-inflammatory phenotype by inhibiting the CD44 function, so that local inflammatory response, oxidative stress and hepatocyte injury of the liver are relieved. Experiments prove that the CD44 function inhibitor can relieve liver inflammation, improve liver function indexes and reduce liver cell death and tissue pathological damage, and a brand-new and targeted scheme for preventing and treating RILD is provided.

The invention discloses a stem cell medicine for pancreas islet repair and application, and belongs to the technical field of stem cell biology, and the stem cell medicine for pancreas islet repair is prepared by compounding a carrier, pancreatic progenitor cells and pancreas islet-like cells in proportion. The problems that in the prior art, due to the fact that stem cells are unstable in differentiation efficiency, poor in synergistic effect and poor in biocompatibility, islet injury cannot be repaired, and diabetes cannot be treated are solved. According to the present invention, the dual mechanisms of endogenous beta cell repair and exogenous beta cell supplement are adopted, and the beta cell function recovery and the group structure reconstruction are synchronously achieved through the reasonable ratio, such that the in-vivo blood glucose of the mouse is significantly reduced, the beta cell number and the glucose tolerance dose of the mouse are increased, and the low immunogenicity and the high anti-inflammatory property are simultaneously provided. The compound can be used for treating type I and type II diabetes mellitus, improving the insulin secretion function and relieving local inflammatory response of pancreas by promoting pancreas islet beta cell proliferation, and has application potential in treatment of type I and type II diabetes mellitus and pancreas islet repair.

The present invention relates to medicine and the pharmaceutical industry, and more particularly to agents capable of inhibiting inflammatory processes, and even more particularly to agents that have an antimicrobial, sorptive, angioprotective and wound-healing effect on local inflammatory processes of the skin and soft tissues, for use in surgery, traumatology and dermatology. What is proposed in the present invention is a combination including dexpanthenol, methyluracil and chloramphenicol, and also a pharmaceutical composition based thereon.

The invention relates to an alkaloid liposome, a preparation process thereof and an application of the alkaloid liposome in an external preparation, and belongs to the technical field of external preparations. The technical problem to be solved is to provide an alkaloid liposome technology which is higher in stability, safe and non-irritating. According to the scheme, the alkaloid liposome technology is characterized in that raw materials comprise alkaloid, hydrogenated lecithin, grease, polyhydric alcohols and water; the alkaloid is selected from at least three of piperine, tetrahydropiperine, tetrahydropalmatine, dihydrooat alkaloid D, caffeine and leonurine; the hydrogenated lecithin is selected from at least one of PHOSPHOLIPON80H or PhoslipTM HPC (Hydroxyethyl Propyl Phosphatidylcholine). The liposome is good in stability and low in irritation, and has the effects of promoting local tissue microcirculation, reducing choked flow rate, increasing local body temperature, enhancing tissue metabolism and improving local inflammation when being externally applied to skin.

The present application relates to the technical field of biological medicine, and particularly relates to Pseudogulbenkiania soli, outer membrane vesicles thereof and application. The present application provides a new method for treating or preventing liver fibrosis and related diseases by using Pseudogulbenkiania soli Pseudochrobactrum saccharolyticum The extracellular membrane vesicles of Pseudogulbenkiania soli and cells derived therefrom can significantly reverse the increase of serum liver function indicators of CCl4-induced liver fibrosis mice, and significantly reduce the histopathological damage of liver tissue. At the same time, both of them can effectively inhibit the local inflammatory response of liver and reduce the expression level of inflammatory factors. The above results show that Pseudogulbenkiania soli and its outer membrane vesicles can significantly improve the liver function damage, histological lesions and inflammatory state caused by CCl4 by regulating the inflammatory signaling pathway, and provide new experimental evidence and potential strategy for microbial intervention of liver fibrosis.

This invention relates to the field of biomedical technology, and in particular to a method for preparing and applying a nanomedicine for treating deep endometriosis. The method involves mixing ferrous ammonium sulfate, trisodium citrate, and polyethyleneimine, then adding thioacetamide and triethanolamine via a solvothermal reaction to obtain two-dimensional iron sulfide nanosheets. Dopamine hydrochloride is then added and stirred at room temperature in anhydrous ethanol to obtain polydopamine-coated iron sulfide (FP). FP is then mixed with larotrectinib and anhydrous ethanol to obtain the nanomedicine FPL for treating deep endometriosis. The synthesis method of this invention is simple and highly operable; the product is stable and reproducible; FPL exhibits good biocompatibility and biodegradability. After entering the microenvironment of deep endometriosis lesions, it can responsively release larotrectinib, effectively inhibiting the Trk signaling pathway and significantly reducing the degree of nerve infiltration; simultaneously, the anti-inflammatory effect of the FP carrier alleviates the local inflammatory response, jointly inhibiting lesion progression.

The invention discloses a full-tumor vaccine for ultrasonic enhancement of immune response and a preparation method thereof. According to the full-tumor vaccine with the ultrasonic enhanced immune response, the manganese vanadate nanosheets with acoustic catalysis and adjuvant effects serve as vaccine adjuvants and acoustic sensitizers, tumor cells taking in the manganese vanadate nanosheets are split to obtain the full-tumor vaccine, under ultrasonic excitation, the manganese vanadate nanosheets can generate ROS at the inoculation position, release of full-tumor cell vaccine antigens is promoted, and the immune response is enhanced. According to the present invention, the manganese vanadate nanosheet can induce local inflammation and promote the collection of innate immune cells and the antigen uptake ability of dendritic cells (DC), and the manganese ions released by the manganese vanadate nanosheet can activate the STING-IFN-I signal channel and promote the maturation of the DC, and after the mature DC uptake the antigen, the mature DC homes to the lymph node to activate the strong antigen-specific T cell immune response, after the full-tumor cell vaccine based on the manganese vanadate nanosheet is injected for a single time, the vaccine can be activated for multiple times as required through external field ultrasound, so that efficient treatment on tumors is realized.

The application discloses application of forskolin and / or isoforskolin in preparation of a medicine for preventing and / or treating colitis, and belongs to the technical field of medicines.The medicine can significantly improve the disease phenotype of colitis, including relieving weight loss, reducing disease activity index score, improving colon length shortening and histopathological damage.The mechanism of action involves multiple targets: repairing intestinal mucosal barrier function, reducing local inflammation of the colon, and regulating intestinal flora balance, restoring the abundance of beneficial bacteria and inhibiting the proliferation of pathogenic bacteria.The application provides a candidate drug with anti-inflammatory, barrier repair and flora regulation functions for the treatment of colitis, and expands the medical use of forskolin or isoforskolin.

The invention relates to the field of medical treatment and health, and discloses a diabetic foot ulcer electrical stimulation and medicine synergistic treatment device and a using method, the diabetic foot ulcer electrical stimulation and medicine synergistic treatment device comprises a flexible main body belt, the two sides of the flexible main body belt are both provided with binding belts, the surface of the left binding belt is provided with a hook face hook-and-loop fastener, and the surface of the right binding belt is provided with a suede face hook-and-loop fastener; and the electrical stimulation drug delivery synergistic structure is used for integrating electrical stimulation and transdermal drug delivery, so that the rehabilitation treatment of the foot ulcer is enhanced. Organic fusion of treatment means is achieved through an electrical stimulation drug delivery synergistic structure, and the limitation of single treatment is broken through: a platinum composite flexible electrode and a gold composite flexible electrode are matched with an electrical stimulation control module, wound surface microcirculation can be improved through low-frequency electrical stimulation, and meanwhile, a blood vessel dilating agent stored in a drug storage chamber can further enhance the microcirculation improvement effect; the anti-inflammatory drug can reduce local inflammatory response possibly caused by electrical stimulation, and the synergistic effect of stimulation synergy and drug side effect reduction is achieved.

The invention discloses a CX3CL1 targeting chimeric antigen receptor-macrophage and application thereof in treatment of GJB2 mutation related hereditary deafness, the CX3CL1 targeting chimeric antigen receptor-macrophage directly converts a CX3CL1 mediated pro-inflammatory signal into an anti-inflammatory signal, so that the macrophage is directionally polarized to an M2 type, the anti-inflammatory repair function is exerted, and the GJB2 mutation related hereditary deafness can be treated by the CX3CL1 targeting chimeric antigen receptor-macrophage. A cochlea injury microenvironment is specifically responded, anti-inflammatory repair phenotypes are switched, and cochlea epithelial cells and spiral ganglion neurons are protected; the GJB2 mutation-related hereditary deafness inner ear local inflammation can be accurately improved, apoptotic cell debris removal is promoted, the whole body immune homeostasis is not interfered, and the GJB2 mutation-related hereditary deafness inner ear local inflammation has a remarkable drug treatment application prospect.

The invention relates to a recombinant protein, printing ink, a 4D scaffold and a preparation method and application thereof, and belongs to the technical field of biology. The recombinant protein provided by the invention can provide extracellular matrix-like structure support, catalyzes generation of dopamine groups, endows the recombinant protein with bionic adhesion performance, and meanwhile can regulate and control the activity of matrix metalloproteinase, maintain balance of scaffold degradation and tissue regeneration, target inflammatory factor genes and relieve local inflammatory response. The recombinant protein, the P (NIPAM-co-AA) copolymer and the KLT peptide-loaded mesoporous SiO2 jointly form the printing ink, and the printing ink can be used for printing a 4D biological scaffold, not only has the effect of the recombinant protein, but also has the effects of temperature responsiveness and promoting cell proliferation and vascularization. Meanwhile, due to the fact that a TPMS structure model is adopted in the 4D stent, directional curling can be achieved, and the better mechanical property is achieved; the hydrogel can be used for tissue repair and filling, tissue regeneration is promoted, and wounds are rapidly healed.

This invention belongs to the field of traditional Chinese medicine preparations, and relates to the preparation method and application of a compound preparation of Yao Yu effervescent tablets with anti-rheumatoid arthritis effect. The compound preparation comprises the following traditional Chinese medicine components: 4 parts by weight of Dioscorea cirrhosa, 3 parts by weight of Tinleaf Vine, 2 parts by weight of Camphor Tree, 2 parts by weight of Rhus chinensis, 3 parts by weight of Rhododendron molle, 1 part by weight of Ephedra sinica, 2 parts by weight of Semi-Acer palmatum, 2 parts by weight of Gynostemma pentaphyllum, and 1 part by weight of Cinnamomum cassia. The preparation steps include: placing Dioscorea cirrhosa, Tinleaf Vine, Camphor Tree, Rhus chinensis, Rhododendron molle, Gynostemma pentaphyllum, Semi-Acer palmatum, Gynostemma pentaphyllum, and Cinnamomum cassia in 6000 parts by weight of 70% ethanol. Extract for 2 hours, repeat three times, filter, and combine the filtrates; concentrate, freeze-dry, pulverize, and pass through an 80-mesh sieve for later use; mix 27.5% freeze-dried powder with 26.45% citric acid, 34.11% sodium bicarbonate, 5.61% PEG6000, and 6.33% lactose evenly, pass through an 80-mesh sieve, and prepare a soft mass using anhydrous ethanol as a wetting agent; pass the soft mass through a 20-mesh sieve and dry at 37℃ for 30 minutes to obtain compound preparation granules; granulate through a 20-mesh sieve for granulation, add lubricant PEG6000 and mix evenly; compress into tablets to obtain compound preparation effervescent tablets. This invention, through pharmacological experiments, confirms that the Yaoyu effervescent tablet compound preparation has a good anti-rheumatoid arthritis effect: it can reduce local inflammatory mediators and serum IL-1β, IL-6, TNF-α, and PEG2 levels, downregulate MDA and increase SOD to improve oxidative stress, inhibit the expression of MMP3, COX-2, and P38 proteins in the synovial membrane, and reduce pathological tissue damage. The medium and high dose groups showed the best effect, and it has a good therapeutic and protective effect on the ankle joint of AA rats, with good clinical application value.

The application provides a traditional Chinese medicine composition for improving digestive system diseases caused by brain-gut disorder, which is made of the following traditional Chinese medicines in parts by weight: Gastrodia 1-35 parts, Astragalus 1-35 parts, Fructus Alpiniae oxyphyllae 1-20 parts, Atractylodes 1-20 parts, Hypericum 1-20 parts, Cinnamomum 1-10 parts, and Dried Ginger 1-10 parts, and further provides a preparation method and application of the composition. The traditional Chinese medicine composition can intervene in the brain-gut disorder related digestive system diseases from the perspective of brain-gut interaction, has the effects of improving anxiety and depression-like behaviors, reducing visceral pain sensitivity, protecting and repairing intestinal mucosal barrier, reducing intestinal local inflammatory response, and regulating intestinal flora structure, so as to realize the comprehensive regulation of emotional abnormalities and digestive system symptoms in brain-gut disorder related digestive system diseases, and has a good clinical application prospect.

The application discloses a kind of silicon dioxide-engineered extracellular vesicle composite nano-preparation for tendon-bone interface repair and its preparation method and application, the preparation includes carrier matrix and functional load component, functional load component is loaded in carrier matrix by the synergistic effect of physical adsorption and channel embedding;The carrier matrix is the bioactive silicon dioxide nanoparticle of core-shell structure, the mesoporous silicon dioxide with central radial channel is in the core, the shell is calcium phosphorus layer, and the nanoparticle surface is modified by bone targeting molecule;Functional load component is the engineered extracellular vesicle of bone marrow mesenchymal stem cell source preprocessed by the bioactive molecule with anti-inflammatory osteogenesis effect.The application is by the dual delivery strategy of " carrier + excretion component", on the one hand, active ion released by silicon dioxide carrier promotes osteogenesis, on the other hand, " high efficiency" exosome is stimulated, and local inflammatory microenvironment is adjusted, and the functional regeneration of tendon-bone interface is accelerated by synergistic effect.

Pharmaceutical formulations for the treatment of crystalline-like retinal degeneration (BCD) comprising a recombinant AAV virus expressing CYP4V2, sodium chloride, poloxamer, phosphate and water for injection, and having a pH of 7.0-7.6. The pharmaceutical preparation has extremely good virus titer stability, extremely few AAV aggregates, high mRNA expression of target genes after multiple freezing and thawing and / or excellent high-temperature stability, animal in-vivo experiments prove that the formula preparation has excellent performance in the aspect of reducing local inflammatory response of retina, and further clinical tests prove that the formula preparation has good clinical application prospects in the aspect of reducing local inflammatory response of retina. The pharmaceutical preparation provided by the invention conforms to the technical specifications of related safety and effectiveness of Chinese pharmacopoeia and United States Pharmacopoeia (USP).

The invention discloses an application of hydrogel loaded with an anti-IFN-gamma antibody in preparation of a medicine for treating diphosphonate related jaw necrosis. Research results show that ZOL treatment can promote gamma delta T cells to secrete IFN-gamma and enhance glycolysis of osteoclasts, lactic acid generated by glycolysis can induce histone H3K18 to be lactylated, a transcription factor IKZF1 and a downstream effector molecule ZBP1 thereof are activated, and finally necrotic apoptosis of the osteoclasts is driven and a bone remodeling function is damaged. The GelMA atab is locally applied to a BRONJ focus, so that controllable release of an anti-IFN-gamma antibody can be realized, the activity of IFN-gamma can be effectively blocked, necrotic apoptosis and local inflammatory response of osteoclast can be remarkably reduced, and BRONJ-like lesions of rats can be improved. Therefore, an effective strategy is provided for treatment of BRONJ, and experimental feasibility support is provided for clinical transformation application of the composite hydrogel.

The application provides a hydrogel millimeter ball with in-situ stress dispersion and a preparation method and application thereof, and belongs to the technical field of biological medicine. The application successfully constructs a millimeter ChS@HM biomimetic hydration millimeter ball based on microfluidic and photocrosslinking technology. The material simulates the structural characteristics of natural NPs, relies on the covalent crosslinking network of ChSMA and HAMA, and realizes the breakthrough of structural bionics and functional synergy. In-vivo and in-vitro experiments verify that the millimeter ball effectively overcomes the limitations of stress concentration and difficulty in maintaining long-term mechanical properties of traditional microspheres due to small size, and significantly improves the stress dispersion capacity of the tissue. At the same time, the anti-inflammatory properties of ChS synergistically alleviate the local inflammatory response, promote the repair of the NP metabolic microenvironment and the recovery of the hydrated state. Overall, the hydration millimeter ball developed by the application exhibits good biological safety and multiple functions, and provides an innovative material strategy and theoretical basis for the regulation of the inflammatory microenvironment and tissue regeneration related to intervertebral disc degeneration.

This invention relates to the field of biomimetic nanozymes and their preparation technology, disclosing a biomimetic nanozyme, its preparation method, and its applications. A peptide-functionalized biomimetic nanozyme (HRPL) is obtained by loading rapamycin onto HMPB nanozymes, coating the surface with a platelet membrane, and functionalizing it with the integrin-binding peptide LXW7. The binding of the platelet membrane to the LXW7 peptide enables targeted delivery to the site of endothelial injury, promoting endothelial repair and re-epithelialization. In an acidic inflammatory microenvironment, HRPL releases rapamycin, inhibiting smooth muscle cell proliferation and migration, and preventing restenosis. HRPL also scavenges reactive oxygen species, reducing oxidative stress and local inflammation, thereby improving the vascular microenvironment. The therapeutic effect was evaluated using a typical rat carotid balloon injury model. This dual-targeting mechanism not only promotes endothelial repair but also reduces restenosis and inflammation, showing significant clinical therapeutic potential. Especially after stent implantation, HRPL helps improve prognosis, reduce complications, and restore vascular homeostasis.

The invention discloses barrier repair type anti-inflammatory hydrogel as well as a preparation method and application thereof, and relates to the technical field of skin repair and inflammation regulation, and the method comprises the following steps: dissolving Cu < 2 + >-containing copper salt in sterile deionized water to obtain a Cu < 2 + > solution, heating, adding xanthan gum, and uniformly mixing to obtain initial hydrogel; sodium hyaluronate, butanediol, glycerol, ceramide, nicotinamide, panthenol and allantoin are added into the initial hydrogel, then the pH value of the obtained system is adjusted to be neutral or weakly acidic, the barrier repair type anti-inflammatory hydrogel is obtained, the ratio of the mass of the copper salt to the hydrogel is (0.01-0.1) g: 100 mL, the action time of active substances is effectively prolonged by stably releasing copper ions, and the barrier repair type anti-inflammatory hydrogel is obtained. The composition can regulate the expression level of local inflammatory factors, improve the problem that the skin is in a high inflammatory state for a long time, improve the local hydration of the skin, and provide a favorable repair environment for a damaged barrier so as to promote the recovery of the structure and function of the skin.

The invention discloses a surface-modified bacillus cereus preparation as well as a preparation method and application thereof, and relates to the technical field of viable bacteria medicines. According to the invention, dopamine and chitosan are modified on the surface of bacillus cereus to form a two-component coating bacillus cereus preparation by utilizing the spontaneous polymerization principle of dopamine and chitosan, and the two-component coating bacillus cereus preparation is used for preparing the medicine for preventing and treating enteritis. Research finds that the bacillus cereus preparation with the polydopamine and chitosan double-component coating can efficiently remove active oxygen to relieve enteritis. The preparation can remain in intestinal tracts of mice for a longer time and has a remarkable curative effect on radiation enteritis caused by 12 Gy gamma ray abdominal irradiation, and compared with an original bacterial liquid group, the weight, intestinal length, histopathology, local active oxygen removal, local inflammation, mucous membrane permeability and the like of the mice given with the preparation are obviously improved in the aspects of weight, intestinal length, histopathology, local active oxygen removal, local inflammation, mucous membrane permeability and the like.

The invention provides application of astaxanthin in preparation of medicines for preventing and treating intestinal dysfunction and intestinal flora imbalance related to severe acute pancreatitis or repairing intestinal mucosal barrier. In-vivo experiments prove that the astaxanthin can significantly improve SAP-induced intestinal barrier injury, reduce the activity of serum diamine oxidase (DAO), repair a jejunum villus structure and up-regulate the expression of tight junction proteins (ZO-1, Occludin and Claudin); the mechanism relates to inhibition of local inflammatory response and oxidative stress, and specific correction of intestinal flora disorder, including recovery of proportion balance of phylum firmicalis and bacteroides, and improvement of abundance of beneficial bacteria such as lactobacillus murine and ruminococcus; the invention provides a new strategy for clinical treatment of SAP complicated with intestinal failure.

The application provides a method for constructing a local inflammation model of an experimental animal brain, which comprises the following steps: anesthetizing the experimental animal and fixing the experimental animal on a positioning device; injecting an immunomodulator into a target region according to the coordinates of the target region by using a puncture tool; controlling the injection speed and dose of the immunomodulator by using a micro-infusion device; keeping the puncture needle in the body for a period of time after injection to reduce the backflow of the liquid medicine; verifying the local immune activation by quantitatively detecting the expression change of an inflammation marker in the target region, and synchronously detecting the expression level of the inflammation marker in a peripheral medium to exclude a systemic immune response. The application can effectively solve the problem that the peripheral inflammation effect is difficult to distinguish in the research of brain diseases caused by systemic administration in the prior art, and can be applied to the research of the local immune activation effect of diseases such as Alzheimer's disease, ischemic stroke and glioblastoma, and the evaluation of the efficacy and mechanism of STING agonists in these diseases.

The application discloses an alkaloid liposome and a preparation process and application thereof in external preparations, and belongs to the technical field of external preparations. The technical problem to be solved is to provide an alkaloid liposome technology with higher stability and safety and no irritation. The scheme points are that raw materials include alkaloids, hydrogenated lecithin, oil, polyhydric alcohol and water; the alkaloids are selected from at least three kinds of piperine, tetrahydropiperine, fumarine, dihydroavenanthramide D, caffeine and leonuride; the hydrogenated lecithin is selected from at least one of PHOSPHOLIPON 80H or Phoslip HPC. The liposome has good stability and low irritation, and has the effects of promoting local tissue microcirculation, reducing resistance, improving local inflammation, improving local body temperature, enhancing tissue metabolism and the like when being used externally on the skin.

The application discloses a POSS-glutathione hybrid material with cartilage protection and a preparation method and application thereof. The hybrid material takes a rigid three-dimensional nanocage structure of a POSS molecule as a core support, glutathione (GSH) molecules are fixed on the surface of the core support through covalent grafting, and a hybrid material with stable structure and persistent function is constructed. The preparation method comprises the following steps: firstly, preparing double-bonded glutathione, and then covalently grafting the double-bonded glutathione with a mercapto POSS (POSS-SH) molecule through an 'ene-thiol click chemistry reaction'. The hybrid material has stable structure, can be effectively enriched at an inflammation site, and improves the bioavailability of GSH; through animal experiments, it is verified that the hybrid material can effectively regulate a local inflammation microenvironment of a joint, significantly reduces the level of proinflammatory factors, thereby plays a cartilage protection role of maintaining cartilage elasticity and promoting tissue homeostasis, and has a good application prospect.