112 results about "Near-Infrared Fluorescence Imaging" patented technology

Endoscopic identification and dissection of a blood vessel in tissue during endoscopic harvest can be enhanced by injecting a bolus of ICG into the bloodstream, illuminating the target vessels with excitation light capable of exciting fluorescence in the ICG, and locating the vessel to be harvested by observing the ICG fluorescence. Moreover, collapsed blood vessel may be harvested by inserting an endoscope into the collapsed blood vessel, injecting into the collapsed blood vessel a bolus containing ICG to re-inflate the blood vessel, illuminating the target vessels with excitation light capable of exciting fluorescence in the ICG; and locating the re-inflated blood vessel by observing the ICG fluorescence.

The invention provides an Au-ICG (gold-indocyanine green) lipidosome multifunctional nano probe and application thereof. The nano probe consists of a kernel and a shell, wherein the kernel is a nanogold particle of which the surface is wrapped with a poly-dopamine layer and adsorbs indocyanine green; the shell is a DSPE-PEG (distearoyl phosphatidyl ethanolamine-polyethylene glycol) lipidosome film which is coupled with lactobionic acid and is chelated with Gd<3+> ions. The Au-ICG lipidosome multifunctional nano probe disclosed by the invention can be used for various image modes such as MRI (nuclear magnetism imaging), CT (computed tomography) imaging and near infrared fluorescence imaging of the liver cancer; a patient only needs to stand medicine supplying of a contrast agent, and various diagnosis effects can be achieved; furthermore, by virtue of the improvement of the sensitivity, the using amount of the contrast agent can be reduced, so that the toxic and side effect is further relieved; under near infrared illumination at 808 nm, adsorbed ICG molecules can effectively convert light energy into heat energy to produce superhigh heat of 60-70 DEG C, so that liver cancer cells can be killed thermally; therefore, the multifunctional nano probe can be used as a multimodal contrast agent for liver cancer diagnosis and can be used as a photothermal therapy agent for the liver cancer.

The invention discloses multifunctional mesoporous silica nanoparticles having near-infrared photothermal and in-vivo fluorescence imaging characteristics as well as a preparation method and an application of the mesoporous silica nanoparticles. Mesoporous silica nanoparticles subjected to amination are modified by carbocyanine dye Cypate, mesoporous channels are loaded with an antitumor drug, the mesoporous silica nanoparticles subjected to amination are prepared with CTAB (cetyltrimethylammonium bromide) as a surfactant and are mixed with the antitumor drug in a solution, so that the mesoporous channels are loaded with the drug, an obtained nano-drug carrier is applied to near-infrared photothermal treatment, near-infrared fluorescence imaging and delivery of the antitumor drug, multi-functionalization of the same nano-drug carrier is realized, the mesoporous silica nanoparticles have very good biocompatibility, and produced chemotherapy and photothermal treatment combined effect is better than that produced with single treatment method.

The invention discloses an Erlotinib-Cy7-chitosan polymer with tumor targeting. According to the Erlotinib-Cy7-chitosan polymer, lung-cancer molecular targeted medicine Erlotinib and the heptamethine cyanine dye Cy7 are connected to chitosan modified by the chemical structure through click chemistry, and a chitosan derivative (CE7) is obtained. According to the Erlotinib-Cy7-chitosan polymer, chemical synthesis is simple and easy to carry out, the polymer CE7 can be self assembled to form nanometer particle CE7Ns, the nontoxic and high-biocompatibility characteristics of chitosan are reserved, the water solubility and the bioavailability of the Erlotinib are greatly improved, the toxic and side effects of medicine are reduced, near-infrared fluorescence imaging and photodynamic therapy can also be carried out, and the therapy effect is improved.

The present invention provides a tumor-targeted nuclear magnetic resonance/fluorescence dual-mode imaging contrast agent, preparation and applications thereof. According to the present invention, the contrast agent is nanometer micro-particles formed by self-assembling a nuclear magnetic resonance amphiphilic molecule, a near-infrared fluorescent dye amphiphilic molecule and a tumor targeting amphiphilic molecule in a solution, can stably exists under a physiological pH value condition, can be specifically accumulated at a tumor site in a targeting manner, can significantly enhance the intensity of the MRI signal and the near-infrared fluorescence signal at a tumor lesion site, can improve the specificity and the sensitivity of nuclear magnetic resonance imaging and near infrared fluorescence imaging scanning, and is expected to be adopted as the contrast agent for fluorescence guidance during tumor resection surgeries and postoperative nuclear magnetic resonance monitoring.

The invention discloses a nano probe material for imaging, which is capable of efficiently breaching blood-brain barriers. Firstly, a high temperature pyrolysis method is adopted for preparing inner core NaYF4:Yb/TM/Gd hydrophobic nano-particles, then an NaGdF4 shell is formed through epitaxial growth so as to form core/casing structured NaYF4: Yb/Tm/Gd and NaGdF4 nano-particles, then hydrochloric acid hydrophilic modification and sulfhydryl PEG modification are performed, and finally, the nano probe material is prepared through Angiopep-2 grafting, and is marked as ANG/PEG-UCNPs. The material can be used for efficient blood-brain barrier breaching, magnetic resonance imaging diagnosis and positioning before an cranial cavity glioma operation, near infrared fluorescence imaging in the operation and image mediating, the imaging effect is good, the sensitivity is high, the physiological system toxicity is low, and the material plays an important significance in successful clinic glioma removing operations and the development and the application of medical image diagnostic techniques.

The invention relates to a preparation method and applications of a photo-thermal and photodynamic co-used antitumor drug delivery system taking gold nano star mediated by folic acid as the carrier. In the prior art, the antitumor drugs have adverse side and toxic effects, moreover, the biocompatibility and targeting performance of the antitumor drugs are bad, and the provided system solves the problems mentioned above. In the preparation method, reaction active amino and carboxylic groups are grafted onto the surface of gold nano stars through covalent bonds, then targeting molecules are connected to the gold nano stars through amidation reactions to obtain a targeting gold nano star solution; then the amino groups on the end of the targeting compounds carry out chemical reactions with near-infrared fluorescence probes so as to obtain a gold nano star solution with functions of folic acid targeting and near-infrared fluorescence imaging; and finally an antitumor drug (adriamycin) is directly loaded on the surfaces of gold nano stars through an electrostatic adsorption effect. The chemical stability and physical stability of the drug delivery system are both very good, the preparation technology is simple, the resources of the raw materials are abundant, the cost is low, the side and toxic effect is little, and the tumor cell proliferation can be effectively inhibited.

The invention relates to a near-infrared fluorescence imaging surgery guide device and an application thereof. The device comprises a near-infrared laser light source, a light collection optical system, a visual light filter, a near-infrared light filter, a color video camera, a near-infrared video camera and a terminal display. The device can conveniently perform position guide and morphic display functions in the process of a lymph excision or inactivation surgery, and meanwhile can be used for analyzing and evaluating the effect of the lymph excision or inactivation surgery. The device is favorable to improving surgery efficiency and accuracy and accumulating surgery treatment experience.

The invention relates to a multifunctional nanomicelle for early diagnosis and phototherapy of tumors. The multifunctional nanomicelle comprises a monolayer functionalized lipid membrane shell, photosensitive molecules and optothermal absorbents, wherein the photosensitive molecules and the optothermal absorbents are embedded into the monolayer functionalized lipid membrane shell. The multifunctional nanomicelle has the advantages that the nanomicelle can be used for multi-modal imaging diagnosis such as early magnetic resonance imaging, photoacoustic imaging or near-infrared fluorescence imaging of cancer; multiple kinds of diagnosis effects can be achieved as long as a patient suffers from one contrast medium administration; since sensitivity is improved, contrast medium injecting dosage is further decreased, and toxic and side effects on the patient can be alleviated; when the tumors are under single-wavelength near-infrared laser radiation, chlorine 6 and Pdots inside the nanomicelle are capable of converting light energy into chemical energy and thermal energy effectively to produce a great number of ROS (reactive oxygen species) oxygen free radicals and overhigh heat at 50-60 DEG C, and the ROS oxygen free radicals and the overhigh heat are used for inducing apoptosis and necrosis of cancer cells, so that a photodynamic and photothermal collaborative therapy function is achieved; the multifunctional nanomicelle not only can be used as a contrast medium for cancer diagnosis, but also can be used as a therapeutic agent for collaborative therapy of the cancer.

Disclosed herein are nanoparticles for diagnosis or treatment of tumors. The nanoparticles include: (a) a core including a Prussian blue dye; and (b) a shell obtained by partially or completely coating a surface of the Prussian blue core with albumin, thereby diagnosing a tumor by a nuclear magnetic resonance imaging apparatus and a near-infrared fluorescence imaging apparatus and necrotizing a tumor by a combined photothermal-photodynamic effect.

The invention discloses a near infrared laser scanning confocal microscopic imaging system. A near infrared laser not contained in an Olympus FV1000 laser scanning confocal microscope is selected as a light source, a near infrared fluorescent probe in a biological specimen is stimulated, near infrared fluorescence is led out by optical fibers and is received by PMT responding to the near infrared light and imaging is preformed. Compared with a traditional laser scanning confocal microscopic imaging system performing stimulation and receiving in visible light bands, the near infrared laser scanning confocal microscopic imaging system has the advantages of smaller biological tissue damage, deeper imaging depth, stable work and reliable performance.

The invention relates to an IR780 double-targeted near-infrared fluorescent probe preparation method and application on tumor diagnosis and treatment, wherein the compound comprises two molecular units including an IR780 mother nucleus and morpholine. In the same molecular structure, mitochondria targeting, lysosome targeting, near-infrared fluorescence imaging diagnosis and photodynamic treatmenton tumors are integrated. The invention also discloses the application on tumor diagnosis and treatment. The fluorescent probe has the advantages of a simple structure, small molecular weight, a determined chemical structure, easy preparation and purification processes, low toxicity to mice and the like, so that the fluorescent probe can meet the basic requirements of clinical medication. In-vitro experiments prove that by means of the near-infrared fluorescence imaging, the electropositivity of tertiary amine and the negative electronegativity of the mitochondria in the near-infrared fluorescence probe structure based on IR780 tumor double targeting can obviously target tumor cells and tumor tissues, can generate photodynamic treatment action under near-infrared radiation, and can obviously inhibit tumor growth.

The present invention relates to a silver sulfide quantum dot-chitosan nanometer complex preparation method, which comprises: 1, dissolving a certain amount of chitosan or a derivative thereof in deionized water at a room temperature to prepare a chitosan aqueous solution with a concentration of 1-20 mg/mL or a chitosan derivative aqueous solution with a concentration of 1-20 mg/mL; 2, under a room temperature stirring condition, sequentially adding a chelating agent and a water soluble silver salt to the chitosan aqueous solution or the chitosan derivative aqueous solution, and carrying out a stirring reaction for 5-20 min; and 3, adding an alkali metal sulfide and a carboxyl compound to the product obtained from the step 2, carrying out a stirring reaction for 5-20 h, carrying out water dialysis and centrifugation on the obtained reaction product, washing sequentially by using absolute methanol and diethyl ether, and carrying out vacuum drying at a room temperature to obtain the silver sulfide quantum dot-chitosan nanometer complex. Compared with the nanometer complex in the prior art, the nanometer complex of the present invention has the following characteristics that: a nanometer complex with a near-infrared fluorescence imaging function is prepared by using a simple and easy-performing method, can be used for biological fluorescence imaging or as a fluorescent probe, and has characteristics of good biocompatibility, no physiological toxicity, and wide application prospects in biomedical fields.

The invention provides a preparation method of a near infrared fluorescence imaging micromolecule anticancer nano-drug. The method comprises the steps of using hydrophobic drugs UA (ursolic acid) andPTX (paclitaxel) for preparing into a nano-micelle, adopting the nano-micelle as a carrier, and co-assembling the carrier and a hydrophilic ICG to obtain the nano-drug. The invention aims to provide the preparation method for preparing the near infrared fluorescence imaging micromolecule anticancer nano-drug through interaction of pi-pi accumulation electrostatic forces of the UA and the PTX withantitumor activity and the photosensitizer ICG, so that the problems that in the prior art, an artificially synthesized carrier nanometer system is complex in system, difficult in quality control, undefined in action mechanism, unclear in metabolism and the like are solved, and the aim of treating tumors through the synergism of phototherapy and chemotherapy is achieved.

The invention relates to a multifunctional nanocapsule integrating near infrared fluorescent imaging with chemotherapy/photothermal therapy and further relates to a preparation method of the multifunctional nanocapsule and application of the same in tumor diagnosis and treatment. A structural schematic diagram of the multifunctional nanocapsule is shown as in the figure, membrane components of themultifunctional nanocapsule include near infrared dye for photothermal therapy, amphipathic Janus drug copolymer for chemotherapy and conventional phospholipid, a proportion of a near infrared absorber to chemotherapy drug can be regulated according to needs, and drug loading capacity is greatly increased. The multifunctional nanocapsule can realize targeted gathering at a tumor position throughEPR effect, so that gathering and intake of drug at the tumor position are improved effectively, and effect on suppressing tumor growth through photothermal therapy and chemotherapy in a combined manner is improved remarkable.

The invention relates to a tumor stem cell marker CD133-targeting polypeptide and an application thereof. A general formula of the polypeptide is CX1X2X3X4X5X6X7X8LX9. The invention further relates to a DNA fragment encoding the polypeptide, an expression vector and host cell expressing the polypeptide, and a bivalent body, a multivalent body or a pharmaceutical composition formed by the polypeptide. The polypeptide is used for detecting and targeting the tumor stem cell, and is good in specificity and sensitivity, simple in preparation method, low in cost and high in practicability. The polypeptide is applied to near-infrared fluorescence imaging to carry out high-sensitivity and high-resolution monitoring and targeted therapy on the CD133 tumor stem cell in a skin transplatation tumor. Simple, fast, economical and accurate detection means can be provided for real-time monitoring of peripheral blood CD133 expression of a patient and the correlation of the CD133 tumor stem cell in tumor recurrence, metastasis, drug tolerance and prognosis, so that the therapeutic scheme is convenient to timely adjust and novel means can be provided for improving the prognosis of patients.

The invention discloses a novel folic acid-chitosan-Cy7 polymer (CF7) serving as a diagnosis and treatment agent and a preparation method of the polymer. 6-nitrine-6-deoxidation-N-phthaloyl-chitosan, alkynyl modified folic acid (ALK-FA) and alkynyl modified heptamethine cyanine Cy7 (ALK-Cy7) react to synthesize a derivative. The polymer CF7 can be self-assembled to form a nano-particle and can be used as the nano diagnosis and treatment agent for tumors, folic acid molecules coupled on a framework can recognize high-expression tumor cells of folic acid receptors in a targeting manner, and Cy7 molecules can be used for near-infrared fluorescent imaging and photodynamic therapy.

According to an exemplary embodiment of the present invention, a versatile delivery module based on a myristoylated polyarginine backbone can be provided to cross a blood-brain barrier (“BBB”). An incorporation of a fatty acid group can be achieved using a Schotten-Bauman reaction with quantitative yield, and a peptide can be further synthesized by conventional solid phase peptide synthesis (“SPPS”). An in vivo distribution of the delivery module into a brain over time using near-infrared (“NIR”) fluorescence imaging can be obtained. A fluorescent cargo can be detected in vivo after an intravenous injection and can be further characterized in perfused brains. A staining of an excised brain can show that the delivery module can primarily accumulate in neurons with occasional localization in astrocytes and endothelial cells. This exemplary approach can be used for the delivery of imaging probes and targeted therapeutics across the BBB.

The invention discloses a handheld fluorescent image navigation positioning device which comprises a handheld shell (1), a power module (2), a micro-projection module (3), an image acquisition module(4), an image processing module (5), an excitation light source module (6) and a distance measuring module (7). According to the device, pathological tissues are developed by the aid of a near-infrared fluorescent imaging technique, ICG (indocyanine green) serves as a contrast medium, acquired fluorescence images directly project to corresponding diseased regions by a real-time projection mode, and projection positions and projection sizes are calibrated by the distance measuring module in real time. Compared with an existing development mode, fluorescent images of the tissues can be more visually observed, the device has higher fluorescent efficiency and better dynamic properties, the whole device is highly integrated as a handheld device, and the device is convenient to operate, can be used for clinical operation and has a good practical prospect.

The invention discloses a preparation method for a biological chip for detecting food-borne pathogenic bacteria and belongs to the technical field of food-borne pathogenic microorganism detection. According to the invention, the unique protein gene sequence of food-borne pathogenic bacteria is utilized to design the specific CP, DP and RCP thereof. CP and DP both contain the sequences complementary with the specific sequence of to-be-detected bacterium genome DNA; the RCP contains no to-be-detected bacterium genome DNA sequences; the CP is fixed on a formyl-modified chip surface in a sample application mode, a capturing probe micro-array is prepared, on-chip CP and DP are annealed and connected with the to-be-detected bacterium genome DNA, streptavidin is added and is combined with a solid-phase RCA amplification product and elution is performed under a certain condition; a near infrared fluorescence imager is used for scanning the chip and is used for detecting if CP and DP are connected with each other and if the rolling circle amplification and target detection can be realized, so that the target food-borne pathogenic bacteria can be specifically detected. The method can be used for detecting various food-borne pathogenic microorganisms and is suitable for high-flux, quick and accurate detection for various food-borne pathogenic microorganisms in food, breeding, ports, and the like.

The invention discloses a photosensitive medicinal preparation containing amino poly-carboxylic acid modification tetraphenylporphyrin compound. Each unit dose contains 1mg-100mg of the amino poly-carboxylic acid modification tetraphenylporphyrin compound. The each unit dosage is the total amount of the medicinal preparation used everyday. The amino poly-carboxylic acid modification tetraphenylporphyrin compound can be used in combination with other medicine or is singly used. Through addition of a drug carrier which is acceptable in pharmacy, the amino poly-carboxylic acid modification tetraphenylporphyrin compound accounts for 0.5%-40% the total weight of the medicinal preparation and can be prepared to any dosage form accepted clinically. The invention further provides application of the medicinal preparation on preparation of photosensitive antitumor drug and in the near infrared fluorescence imaging filed. The photosensitive medicinal preparation containing the amino poly-carboxylic acid modification tetraphenylporphyrin compound is low in toxicity, convenient to apply, wide in tumor suppression spectrum, good in tumor control rate, good in effect of malignant tumor treatment through photodynamic therapy, high in fluorescence quantum yield and optical stability in a near-infrared area, good in biocompatibility, capable of having a certain ability of penetrating the biological barrier, and wide in application prospect in the near infrared fluorescence imaging field.

The invention discloses a polymerizable NIR (near infrared) fluorescent dye monomer as well as a preparation method and application thereof. The NIR fluorescent monomer (NFM) is synethized by firstly reacting a fluorine-boron fluorescent dye with methacryloy chloride and then purifying. The synthetized new NFM provides a new method for controllable synthesis and application of NIR fluorescent polymers and has wide application prospect in in-vivo NIR fluorescent imaging.

The invention belongs to the technical field of medicine, and particularly relates to a multifunctional composite nanometer medicine for tumor imaging and treatment and a preparation method of the composite nanometer medicine. The composite nanometer medicine comprises an active component-1, an active component-2 and a stabilizing agent, wherein the active component-1 includes an antitumor drug of sorafenib or a derivative thereof, the active component-2 includes a near infrared fluorescent component of indocyanine green or a derivative thereof, and the stabilizing agent includes Pluronic, PLGA-PEG, TPGS, DPSE-mPEG, PVA, Tween, PEG, and Lipid. The composite nanometer medicine can be obtained through a simple and convenient nanometer precipitation method and has the size of 50-800 nm. The composite nanometer medicine has multiple functions of tumor chemotherapy and photo-thermal/photodynamic therapy, and indocyanine green is used for near infrared fluorescence imaging. The composite nanometer medicine is excellent in tumor targeting. The two components have an obvious synergistic effect, thereby improving the tumor treatment efficiency, and can be applied to tumor treatment and fluorescence imaging.

The invention relates to the technical field of photothermal nanomaterials, particularly tumor-targeting nanoparticles, a preparation method and application thereof, the tumor targeting nanoparticleshave a spherical shell-core structure, comprises a PFOB core and an IR780 core liposome membrane surrounding the PFOB core, wherein the IR780 core liposome membrane comprises a liposome layer and IR780 iodide particles, and the tumor-targeting nanoparticles have a particle size range of 220 to 320 nm. The potential of the tumor targeting nanoparticles is-28.32- -19.48 mV, drug loading 3.87%-4.87%and encapsulation efficiency 85.7%-87.9%. The invention provides a tumor-targeting nanoparticle, preparation method and application thereof, which can evaluate tumor by preoperative photoacoustic imaging, CT imaging and near-infrared fluorescence imaging, optimize tumor resection under the guidance of intraoperative near-infrared fluorescence, and kill residual focus by auxiliary photothermal treatment during operation.

The invention discloses a preparation method and application of a tumor-targeted diagnosis and treatment combined single-molecule lead compound, and the lead compound is prepared from a terpene-mothernucleus, aryl nitrogen mustardand N, N-diethyl tertiary nitrogen, wherein the structure is shown in (I) as shown in the specification, and mitochondria targeting, fluorescence imaging diagnosis, photodynamic therapy and chemotherapy are integrated in the same molecular structure. The lead compound has the prominent characteristics of being simple in structure and small in molecular weight, determined in chemical structure, easy to prepare, purify and further modify, low in the toxicity to mice, and the like, and the basic requirements of clinical medication are met. An in-vitro experiment proves that tertiary amine electropositivity and amitochondrial electronegativity found by near infrared fluorescence imaging of the lead compound structure remarkably target tumor cells and tumor tissues, photodynamic therapy effects can be generated under near-infrared radiation, and the tertiary amine electropositivity and amitochondrial electronegativity and the nitrogen mustard in the lead compound structure can generate chemical treatment and synergistic treatment, so that the growth of tumors can be remarkably inhibited.