The invention relates to a formula and a preparation method of a Huichunganlu tonifying wine. According to the present invention, the Huichunganlu tonifying wine comprises the aboveground part of equisetum arvense, the root bark of aralia chinensis, the root bark of eleutherococcus senticosus, the dried root of morinda officinalis how, the dried and mature fruit of schisandra chineisis (turcz.) baill, the tender leaf of pinus massoniana lamb, the mature seed of elaeagnus pungens, the mature fruit of ligustrum lucidum ait, the ratan of millettia dielsiana, baijiu and the like, flavoring and aroma increasing is performed with maltose, corn popcorn and the bud of rosa banksiae ait. var. normalis regel, and purities are removed with egg white; the unique preparation method comprises: selecting clean Chinese herbs, cutting, crushing, pressing, soaking, filtering, carrying out water bath heating and aroma increasing, removing purities, blending, carrying out fine filtration, sub-packaging, and the like; the aroma is extracted with the natural bud of rosa banksiae ait. var. normalis regel and the corn popcorn through the water bath heating without the addition of other non-natural ingredients, such that the unique natural aroma is provided and the test results show that the Huichunganlu tonifying wine has effects of immunity improving, bone and muscle strengthening and aging delaying, is suitable for long-term use, and is safe and effective.