The invention discloses ganoderma lucidum wine with functions of soothing liver, regulating qi and increasing primordial qi and a preparation method of the wine. The wine is prepared from raw materials in parts by weight as follows: 8-12 parts of ganoderma lucidum, 4-8 parts of semen euryales, 2-6 parts of rhizoma polygonati, 0.5-1.1 parts of fructus momordicae, 4-8 parts of fructus crataegi, 2-4 parts of radix notoginseng, 6-10 parts of fructus lycii, 4-8 parts of rhizoma drynariae, 4-8 parts of fructus mori, 4-8 parts of poria cocos, 4-8 parts of fructus hippophae, 2-6 parts of fructus alpiniae oxyphyllae, 3-7 parts of fructus rubi and 800-1,200 parts of rice wine. The wine is prepared after the traditional Chinese medicines are soaked in the rice wine, the medicines have good synergistic and curative effects, have the functions of soothing the liver, regulating qi, strengthening the spleen and the stomach, removing food retention, helping digestion, nourishing yin and the kidney, promoting blood circulation to remove blood stasis, tonifying the kidney and the spleen, increasing the primordial qi, dispelling wind, eliminating dampness, relaxing tendons, activating collaterals and relieving swelling and pain and are safe, reliable and free of toxic and side effects.