39results about How to "No high temperature and high pressure reaction" patented technology

The invention discloses a reverse demulsifier and its preparation method. The method comprises allowing reaction between methyl acrylate and ethylenediamine to obtain linear polyamidoamine, and allowing reaction between the linear polyamidoamine and epoxy chloropropane to obtain quaternary ammonium salt of linear polyamidoamine; allowing free-radical polymerization between polyoxyethylene nonylphenyl ether acrylate, acryloyloxyethyltrimethylammonium chloride and acrylamide in the present of an initiator and a terminator to obtain amphipathic cationic polymer; and mixing the quaternary ammonium salt of linear polyamidoamine, the amphipathic cationic polymer, a cationic surfactant and water to obtain the reverse demulsifier. The inventive reverse demulsifier has good demulsification effect on oil-in-water emulsion produced from oil field, and is suitable for chemical flooding produced fluid; and the inventive preparation method has low requirement for equipment, and is free of high-temperature high-pressure reaction.

The invention relates to a method for preparing a rubber auxiliary agent, and particularly discloses a method for preparing rubber peptizer 2,2-dibenzoylamino diphenyl disulphide (DBD). The method for preparing the rubber peptizer DBD is characterized by comprising the following steps of: performing ring opening reaction on benzothiazole to obtain a ring opening product; oxidizing the ring opening product to prepare diphenylamine disulphide; and performing acylation reaction on the diphenylamine disulphide and benzoyl chloride to generate the DBD. According to the method for preparing the rubber peptizer DBD, compared with the conventional process, the product cost is remarkably reduced; the method is favorable for popularization and application of the environmentally-friendly peptizer DBD, and simultaneously breaks through the situation that the product depends on import for a long term; the organic solvent is used as a reaction medium and can be recycled, so little three-waste is discharged; and the production flow is simple and easily controlled, high-temperature and high-pressure reaction does not exist in the method, the equipment investment is low, and the method is easy for production and operation.

The invention relates to a method for synthesizing glutaric acid from alpha-ketoglutaric acid. Specifically, under the mild condition, protected ketone thioacetal is subjected to catalytic hydrogenation reduction with raney nickel, recycling and reusing of reactants, solvent and a catalyst are achieved in a carbonyl reduction part in the most critical step, and the method for preparing the largely-demanded glutaric acid with the simple four-step method is developed. According to the method, the glutaric acid with the high yield is synthesized with the method for the first time, the yields of all steps are 92%, 78.6%, 90% and 85% in sequence, and the total yield is 55.32%; the method has broad prospects in synthetic application of compound library establishment.

The invention discloses a preparation method of a Buvaracetam intermediate shown in B-VI, comprising the following steps: dissolving B-IV and R-phenethylamine in a solvent, crystallizing, filtering, and recrystallizing to obtain B ‑V, which converts to B‑VI. In the preparation process of the present invention, no chiral chromatographic column is required to separate the isomers, and the effective components can be separated only by simple steps such as extraction, washing, drying, concentration, etc., the separation process is simple, and the production cost of brivaracetam is greatly reduced.

The invention discloses a synthetic method of [(trifluoromethyl)thio]benzene compounds, particularly a method for converting substituted phenyl diazonium salts into [(trifluoromethyl)thio]benzene. Themethod comprises the following steps: under white light irradiation, adding an aryl diazonium salt, tris(2,2'-bipyridine)ruthenium(II) hexafluorophosphate and S-trifluoromethyl-4-methoxybenzenesulfonyl sulfate into a solvent and uniformly mixing the mixture; after mixing, reacting the mixture at the room temperature of 20-25 DEG C; determining the reaction ending time through spotting detection;after the reaction is finished, adding water for extraction; performing separation to obtain a liquid organic phase; drying the liquid organic phase with anhydrous Na2SO4; then removing a solvent; andperforming rapid separation with column chromatography to obtain a [(trifluoromethyl)thio]benzene compound. The method is mild in reaction condition, free of high-temperature and high-pressure reaction, safe in reaction condition and suitable for large-scale production and development.

The invention discloses a kind of Aggeria, S ‑Equol-producing engineering bacteria and its construction method and application relate to the field of microbial technology. Eggeria HAU‑JLC44, the preservation number is CGMCC No.12354. S The construction method of equol-producing engineering bacteria is as follows: three invertase genes involved in equol synthesis are cloned from Eggeria HAU-JLC44: E‑dgr , E‑dhdr and E‑thdr ;Will E‑ dhdr , E‑thdr and the 145 bp non-coding region sequence between the two genes were cloned as one gene, called E‑tdhdr ;Will E‑ dgr and E‑tdhdr Cloned to pETDuet‑1, expressed in BL21 (DE3), to obtain engineering bacteria. The present invention uses Escherichia coli engineering bacteria co-expressing three genes to convert the substrate daidzein or daidzein into S ‑Equol, with stable conversion performance and simple method.

The invention belongs to the field of radiochemical synthesis, and more specifically relates to radioisotope carbon-14 labelled pesticide chlopyrifos, and a synthesis method thereof. The synthesis method comprises following steps: a metal organic reagent is reacted with [14C] carbon dioxide so as to obtain 5-benzyloxy[carbonyl-14C] pentanoic acid; amidation, debenzylation, and cyclization reactionare adopted so as to realize conversion into [carbonyl-2, 6-14C2] glutarimide; aromatization, chlorination, and hydrolysis are adopted, and an obtained hydrolysate and diethyl thiophosphoryl chlorideare subjected to nucleophilic substitution so as to obtain the carbon-14 labelled pesticide chlopyrifos. The synthetic method is capable of avoiding defects in the prior art; the reaction raw materials are easily available; the reaction conditions are mild; production cost is low; the obtained carbon-14 labelled pesticide chlopyrifos can be taken as a radioactive tracer, can be used in tracing ofpollution evolution rules such as migration conversion, metabolism degradation, and absorption enrichment of chlopyrifos in the environment and in vivo, and can be used for providing technical support for comprehensive evaluation of the biological safety of chlopyrifos; and in addition, the synthesis method is capable of providing reference for preparation of building blocks containing C-14.