The application discloses a kind of relieving
physical fatigue compositions and preparation method thereof, belong to
functional food and
enzyme engineering technical field;For the technical contradiction that
tannic acid astringency is heavy in existing
Paraguay tea beverage, and acidic environment is easy to lead to
ginsenoside Rb1
hydrolysis inactivation, a kind of step temperature-pH
coupling immobilized enzyme catalysis synergistic treatment method is proposed;The method first uses C8-C18
alkyl hydrophobic modification
mesoporous silica fixed
tannin enzyme, under pH 5.0-6.0, 40-50 DEG C, high-efficiency astringency is removed;Subsequently under the condition that
enzyme preparation is not separated, in situ pH is adjusted to 6.5-7.0 and temperature is reduced to 30-35 DEG C, then
ginseng extract is added to carry out low-temperature protection reaction, and the destruction of
ginsenoside Rb1
glycosidic bond by acid / heat is avoided from the root.The experiment shows that the application can make
tannic acid removal rate ≥72.5%,
ginsenoside Rb1
retention rate ≥82.5%, and
caffeine retention rate ≥86.9%, which is significantly better than traditional process.