64 results about "Phytenic acid" patented technology

The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.

This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and/or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The invention further provides phytases which have increased gastric lability. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate.

The invention relates to the field of gene engineering, in particular to an optimized and improved escherichia coli phytase APPA-M with enhanced catalytic activity in acidic range, and gene and application of the optimized and improved escherichia coli phytase APPA-M. For improving the catalytic capability and the specific activity of the escherichia coli phytase APPA in acidic range, 22 key amino acids in active center of the escherichia coli phytase APPA are subjected to saturation mutagenesis by utilizing gene engineering means. When mutational sites are M216C and N306D, the catalytic activity of the optimized and improved escherichia coli phytase APPA-M in acidic range of pH2-5 is greatly improved, and a huge application potential can be showed in application.

The present invention relates to the use of naturally occurring methylated branched chain fatty acids, precursors, or derivatives such as but not limited to 3,7,11,15-tetramethyl-2-Hexadecen-1-ol (phytol), 3,7,11,15-tetramethylhexadec-2-enoic acid (phytenic acid), 3,7,11,15-tetramethylhexadecanoic (phytanic acid) or 2,6,10,14-tetramethylpentadecanoic (pristanic acid) as amino acid salts for cosmetic, dietary supplement, or pharmaceutical compositions, which are useful in increasing the variables associated with physical performance for the regulation of athletic function.

Belonging to the technical field of minimally invasive rust removal on metal surfaces, the invention, more specifically, relates to an environment-friendly rust remover. The rust remover is characterized in that: corrosion at places that cotton swabs, cloth strips, and cotton threads can reach can all be removed, and the rust remover also has a short-term antirust function, so that enough time can be acquired for long-acting anticorrosion treatment. The rust remover is especially suitable for corrosion and rusty spots appearing on some precision equipment, instruments and meters, and processed precision parts and components. Additionally, for the corrosion problem of some electroplated parts, the rust remover also can reach very good surface treatment effects. The rust remover disclosed in the invention is composed of the following components by mass: 5-15% of tartaric acid, 10-20% of citric acid, 1-5% of phytic acid, 1-5% of sodium salicylate, 0.1-0.5% of a surfactant OP-10, and the balance distilled water.

The invention discloses phytic acid modified transition metal layered double hydroxide as well as a preparation method and application thereof, and belongs to the technical field of preparation of electrolytic water anode electrode materials. The preparation method comprises the following steps: sequentially dissolving transition metal salt and an alkaline substance in a solvent, uniformly mixing, adding a pretreated conductive substrate, and growing transition metal layered double hydroxide on the surface of the conductive substrate in situ by adopting a hydrothermal method or a solvothermal method; and immersing the conductive substrate with the transition metal layered double hydroxide growing on the surface into a phytic acid solution, and preparing the phytic acid modified transition metal layered double hydroxide through a solvothermal method. The preparation method disclosed by the invention is simple and easy to implement and mild in reaction condition. The prepared phytic acid modified transition metal layered double hydroxide is used as an electrocatalyst for water oxidation reaction, and shows excellent catalytic activity and durability in alkaline electrolyte.

The invention discloses a preparation method of a persimmon coating preservative. The preparation method comprises the steps of weighing 5-15% of pectin, 2-5% of D-erythorbic acid. 10-20% of chitosan, 0.1-2% of natamycin, 5-10% of phytic acid, 5-12% of citric acid, 15-25% of ethanol and the balance of water according to a formula, and mixing to obtain the persimmon coating preservative. The preparation method has the beneficial effects that the preservative is safe and nontoxic, broad-spectrum antibacterial and bactericidal effects are good, the moisture evaporation can be well prevented, the antioxidant performance is excellent, the fabrication cost is low, and the operation is simple.

The invention provides phytase from aspergillus fumigatus, and creates a trichoderma reesei engineering strain capable of recombining and expressing the phytase by introducing phytase genes into trichoderma reesei. The engineering strain can efficiently express the phytase, and the shake-flask fermenting enzyme activity is 880 U/mL. The phytase prepared by the invention can be widely used in feedstuff, can greatly improve the average daily weight gain and the feed conversion ratio in each stage of cultivated animals, and reduces the phosphorus excretion by 13.3%, thereby facilitating increase of cultivation benefits and reducing environmental contamination.

The invention discloses a photosensitive phytic acid doped polyaniline-based ultraviolet curing anticorrosive coating and a preparation method thereof, and belongs to the technical field of anticorrosive coatings. Photosensitive phytic acid is prepared through a polymerization reaction of phytic acid and glycidyl methacrylate, the photosensitive phytic acid is used as a doping agent, a double-bond structure capable of participating in a photopolymerization process is introduced while polyaniline doping modification is completed, photosensitive polyaniline is obtained, and the photosensitive polyaniline is applied to an ultraviolet curing coating layer. By doping the polyaniline with the photosensitive phytic acid, on one hand, the problem of poor compatibility of polyaniline and resin can be improved, and on the other hand, double-bond groups can participate in the prepolymer curing and crosslinking process in a photocuring system, so that the crosslinking density of the ultraviolet curing coating layer is improved. Meanwhile, phosphate groups in the photosensitive polyaniline can act with a base material, and the mechanical anchoring effect is improved through chemical bond generation, so that the defect of insufficient adhesive force caused by large instantaneous reaction shrinkage stress in the photopolymerization process is overcome.

The invention provides boron-phosphorus co-doped activated carbon for adsorbing aromatic-ring-containing compounds in water and a preparation method of the boron-phosphorus co-doped activated carbon,and belongs to the technical field of treatment of organic pollutants in water. The preparation method comprises the steps of uniformly mixing boric acid, phytic acid, a carbohydrate and water, and sequentially carrying out hydrothermal reaction, drying, calcining, water washing and drying on the obtained product to obtain the boron-phosphorus co-doped activated carbon. According to the preparation method, boric acid and phytic acid are used as pore forming agents together, so that the preparation cost can be reduced, and the advantage of in-situ pore forming is maintained. Boron doping and phosphorus doping are introduced into the boron-phosphorus co-doped activated carbon at the same time, and the electron donating capacity of phosphorus counteracts the reduction of aromatic ring chargedensity caused by boron doping, so that the intensity of the EDA effect is maintained, and the adsorption performance of the activated carbon on aromatic ring-containing compounds in the adsorption application of the aromatic ring-containing compounds in water is improved.

The invention relates to the field of genetic engineering, in particular to a low-temperature neutral phytase PhyH with double structure domains as well as a gene and application thereof. The invention provides the neutral phytase PhyH from Bacillus sp.HJB17, and the amino acid sequence of the neutral phytase PhyH is shown as SEQ ID NO.1; and the invention provides a gene PhyH for coding the neutral phytase. The phytase provided by the invention has good pH stability and still keeps higher enzyme activity in the alkaline range; the phytase has higher relative enzyme activity in a low-temperature range; the phytase has double structure domains, an N terminal is an incomplete BPP (Butyl Pivalate Peroxide) phytase structure domain and a C terminal is a complete phytase structure domain. In short, the PhyH obtained by the invention can be used as the low-temperature neutral phytase which can be applied to cultivation of fish; the PhyH-DI structure domain can increase the catalytic efficiency of other phytases and is good for improving the production and the application of the other phytases.

The invention belongs to the technical field of microbiological engineering modification, and specifically provides a Trichoderma reesei mutant strain for highly producing temperature-resistant phytase and application of the Trichoderma reesei mutant strain. A preservation number of Trichoderma reesei is CCTCC NO:M2018836. The Trichoderma reesei mutant strain is capable of efficient recombinant expression of the phytase, the enzyme activity of the phytase in shake flask fermentation liquid reaches up to 1950U/ml and is increased by 54.5% than that before mutation, and the enzyme activity of the phytase in fermented supernate of a 20L tank reaches up to 16680U/ml and is increased by 49.8% than that of an original strain. The heat resistance of the phytase obtained from recombinant expression of the Trichoderma reesei mutant strain is remarkably increased, and the phytase has very high enzyme activity and can be widely applied to the feed production field.

The present invention relates generally to methods of use and compositions useful to reduce dark spots, age spots, and/or unwanted pigmentation of skin. The composition includes a combination of niacinamide, phytic acid, Rosmarinus officinalis leaf extract, Chondrus crispus extract, and a combination of Saxifraga sarmentosa extract, Carica papaya (papaya) fruit extract, and Psidium guajava (guava) fruit extract. The composition is effective to reduce dark spots, age spots, and/or unwanted pigmentation of skin. The composition is effective to inhibit tyrosinase and/or Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ) activity. The composition is further effective to reduce melanin content in skin, particularly dark skin, in some instances by at least 30%.

The invention discloses a novel hydrogel electrode patch for health monitoring and a preparation method thereof, wherein the specific preparation method comprises the steps: dissolving glycerin, gelatin, polyvinyl alcohol and phytic acid in water, converting the mixture into a clear transparent solution in an environment of 70-100 DEG C, pouring the transparent solution into a mold for freezing treatment, and thus obtaining the novel hydrogel electrode patch for health monitoring. The novel hydrogel electrode patch disclosed by the invention is colorless and transparent, has good spatial stability, water locking property, electrical conductivity, antibacterial property, adhesiveness and easy complete stripping property, and has a good application prospect in the aspect of human-computer interaction medical monitoring equipment.

The invention discloses a zinc phytate@zeolite imidazate skeleton-8/molybdenum disulfide nanocomposite and a preparation method and application thereof, and belongs to the technical field of anti-corrosion coatings. Polydopamine modified molybdenum disulfide is used as a template, and zinc phytate@zeolite imidazate skeleton-8 nanoparticles are grown in situ on the surface of the template. Zinc phytate@zeolite imidazate skeleton-8 is evenly distributed through the lamellar structure of molybdenum disulfide, the smoothness of the composite coating is effectively improved, the corrosion rate of a metal base material is reduced, the zinc phytate@zeolite imidazate skeleton-8 nanoparticles on the surface of molybdenum disulfide can be stimulated by a surrounding corrosive medium to be cracked, and the corrosion inhibitor zinc phytate and 2-methylimidazole are released. A metal anti-corrosion emulsion prepared on the basis is a nano composite material with passive and active protection functions, and the anti-corrosion performance of a polyacrylate coating is synergistically improved.

The invention belongs to the technical field of composite materials, and particularly relates to a phytic acid-vanadium pentoxide composite material, a preparation method thereof, an electrode and a battery. The composite material is a lamellar material, and phytic acid coats the outer surface of vanadium pentoxide and is intercalated between adjacent layers of vanadium pentoxide. The composite material can effectively overcome/improve the problems of relatively poor specific capacity and cycle performance caused by difficult deintercalation of zinc ions and poor structural stability in a cycle use process when the existing vanadium pentoxide is used as an aqueous zinc ion battery positive electrode material.

The invention relates to the field of genetic engineering, in particular to a low-temperature neutral histidine phytase APPA206, gene thereof, a recombinant carrier comprising the gene and application. The amino acid sequence of the low-temperature neutral histidine phytase APPA206 is shown as SEQ ID NO.1, and the invention also provides the gene appa 206 which encodes the low-temperature neutral histidine phytase. The low-temperature neutral histidine phytase is obtained, has high enzymatic activity at the pH range of 4.5 to 8.5, and can resist hydrolysis of trypsin in an aquatic animal body. The characteristics are suitable for a freshwater fish feed.