66 results about "Phytenic acid" patented technology

The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and / or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.

The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and / or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.

In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.

This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and / or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.

The invention relates to a chromium-free passivating solution for plated metal and a plating method thereof. The passivating solution comprises 0.001 to 30 g / L of vanadate and 0.01 to 30 g / L of molybdate, and can further comprise an aqueous solution of one or more selected from the group consisting of 1 to 300 g / L of phytic acid, 0.01 to 30 g / L of silane and 1 to 500 g / L of a resin. The passivating solution can be used for plated metal through immersion, spraying, roller coating or brush painting and forms a replacing layer and / or a covering layer on the surface of treated metal, which enables surface anti-corrosion performance of treated metal to be effectively enhanced; in addition, the passivating solution provided in the invention does not contain environment-polluting chromium of any valence state, which is beneficial for environmental protection.

The invention relates to the genetic engineering field, especially to a land bacillus Pedobacter sp. MJ11 with a preservation number of CGMCC No. 2503 and a neutral phytase PHYMJ11, a gene thereof, a recombinant vector containing the same and its application. The invention provides the neutral phytase PHYMJ11 with an amino acid sequence shown as SEQ ID NO. 1 and a gene phyMJ11 for coding said neutral phytase. The neutral phytase obtained in the invention has the optimum pH value 7. 0 and simultaneously has a high enzymatic activity in a range of pH5-9. The ability for hydrolyzing a phytate phosphorus in a bean pulp of the phytase in a neutral condition is better than the present used phytase, which enables the phytase in the invention to apply to a fresh water fish feed stuff.

This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The invention further provides phytases which have increased gastric lability. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate.

The invention relates to the field of gene engineering, in particular to an optimized and improved escherichia coli phytase APPA-M with enhanced catalytic activity in acidic range, and gene and application of the optimized and improved escherichia coli phytase APPA-M. For improving the catalytic capability and the specific activity of the escherichia coli phytase APPA in acidic range, 22 key amino acids in active center of the escherichia coli phytase APPA are subjected to saturation mutagenesis by utilizing gene engineering means. When mutational sites are M216C and N306D, the catalytic activity of the optimized and improved escherichia coli phytase APPA-M in acidic range of pH2-5 is greatly improved, and a huge application potential can be showed in application.

The invention discloses a method for preparing an electrochemical biosensor based on a horse radish peroxidase-porous zirconium phytate nanoparticle modified glassy carbon electrode. The method comprises the following steps of: dripping a ZrOCl2 acidic solution into a sodium phytate solution and reacting for 1h; cooling and then centrifuging, and dispersing by using ultrapure water to prepare nano porous zirconium phytate colloid; dripping an appropriate amount of zirconium phytate micelle dispersing solution on the surface of a polished glassy carbon electrode and airing; dripping a horse radish peroxidase solution on a nano zirconium phytate modified glassy carbon electrode, and airing; and dripping an appropriate amount of perfluorinated sulfonic acid solution, and airing to obtain theglassy carbon electrode. The invention has the advantages that: the zirconium phytate colloid dispersing solution with the porous structure is prepared and to be used for fixing biological enzymes, keeps the bioactivity of the enzymes and realizes the direct electron transfer between the enzymes and the electrode; the preparation method is simple; and the response time to the hydrogen peroxide detection is shorter, the detection limit is low, and the repeatability and the stability are good.

The invention belongs to the technical field of material preparation and photocatalysis and in particular relates to a preparation method for carbon nitride nanofibers and an application thereof. Thepreparation method comprises the following steps: separately preparing melamine and phytic acid into aqueous solutions, mixing the aqueous solutions in a certain proportion and uniformly mixing the aqueous solutions; putting the mixed solution in an ice-water bath for quick cooling, standing still to prepare a melamine / phytic acid hydrogel, and drying the melamine / phytic acid hydrogel to obtain amelamine / phytic acid dry gel by using a lyophilization method; and calcining the melamine / phytic acid dry gel to prepare the carbon nitride nanofibers. The preparation method for carbon nitride nanofibers employing an ultra-molecular assembling method is quick and simple, easily available in raw material, low in cost and environment-friendly in preparation process, and the prepared material is uniform in shape, large in specific surface area and good in photocatalytic performance. The rate of photocatalytic water splitting to generate hydrogen is 10 times of that of a common phase carbon nitride.

The present invention relates to the use of naturally occurring methylated branched chain fatty acids, precursors, or derivatives such as but not limited to 3,7,11,15-tetramethyl-2-Hexadecen-1-ol (phytol), 3,7,11,15-tetramethylhexadec-2-enoic acid (phytenic acid), 3,7,11,15-tetramethylhexadecanoic (phytanic acid) or 2,6,10,14-tetramethylpentadecanoic (pristanic acid) as amino acid salts for cosmetic, dietary supplement, or pharmaceutical compositions, which are useful in increasing the variables associated with physical performance for the regulation of athletic function.

Belonging to the technical field of minimally invasive rust removal on metal surfaces, the invention, more specifically, relates to an environment-friendly rust remover. The rust remover is characterized in that: corrosion at places that cotton swabs, cloth strips, and cotton threads can reach can all be removed, and the rust remover also has a short-term antirust function, so that enough time can be acquired for long-acting anticorrosion treatment. The rust remover is especially suitable for corrosion and rusty spots appearing on some precision equipment, instruments and meters, and processed precision parts and components. Additionally, for the corrosion problem of some electroplated parts, the rust remover also can reach very good surface treatment effects. The rust remover disclosed in the invention is composed of the following components by mass: 5-15% of tartaric acid, 10-20% of citric acid, 1-5% of phytic acid, 1-5% of sodium salicylate, 0.1-0.5% of a surfactant OP-10, and the balance distilled water.

The invention discloses phytic acid modified transition metal layered double hydroxide as well as a preparation method and application thereof, and belongs to the technical field of preparation of electrolytic water anode electrode materials. The preparation method comprises the following steps: sequentially dissolving transition metal salt and an alkaline substance in a solvent, uniformly mixing, adding a pretreated conductive substrate, and growing transition metal layered double hydroxide on the surface of the conductive substrate in situ by adopting a hydrothermal method or a solvothermal method; and immersing the conductive substrate with the transition metal layered double hydroxide growing on the surface into a phytic acid solution, and preparing the phytic acid modified transition metal layered double hydroxide through a solvothermal method. The preparation method disclosed by the invention is simple and easy to implement and mild in reaction condition. The prepared phytic acid modified transition metal layered double hydroxide is used as an electrocatalyst for water oxidation reaction, and shows excellent catalytic activity and durability in alkaline electrolyte.

The invention provides phytase from aspergillus fumigatus, and creates a trichoderma reesei engineering strain capable of recombining and expressing the phytase by introducing phytase genes into trichoderma reesei. The engineering strain can efficiently express the phytase, and the shake-flask fermenting enzyme activity is 880 U / mL. The phytase prepared by the invention can be widely used in feedstuff, can greatly improve the average daily weight gain and the feed conversion ratio in each stage of cultivated animals, and reduces the phosphorus excretion by 13.3%, thereby facilitating increase of cultivation benefits and reducing environmental contamination.

This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The invention further provides phytases which have increased gastric lability. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate.

The invention discloses a photosensitive phytic acid doped polyaniline-based ultraviolet curing anticorrosive coating and a preparation method thereof, and belongs to the technical field of anticorrosive coatings. Photosensitive phytic acid is prepared through a polymerization reaction of phytic acid and glycidyl methacrylate, the photosensitive phytic acid is used as a doping agent, a double-bond structure capable of participating in a photopolymerization process is introduced while polyaniline doping modification is completed, photosensitive polyaniline is obtained, and the photosensitive polyaniline is applied to an ultraviolet curing coating layer. By doping the polyaniline with the photosensitive phytic acid, on one hand, the problem of poor compatibility of polyaniline and resin can be improved, and on the other hand, double-bond groups can participate in the prepolymer curing and crosslinking process in a photocuring system, so that the crosslinking density of the ultraviolet curing coating layer is improved. Meanwhile, phosphate groups in the photosensitive polyaniline can act with a base material, and the mechanical anchoring effect is improved through chemical bond generation, so that the defect of insufficient adhesive force caused by large instantaneous reaction shrinkage stress in the photopolymerization process is overcome.

The invention belongs to the technical field of preparation of flexible self-supporting supercapacitors, and discloses a preparation method and application of a self-supporting flexible polyaniline supercapacitor material. The preparation method comprises the following steps: preparing a polyaniline conductive polymer from PVA, phytic acid and triaminobenzeneboronic acid serving as dopants and cross-linking agents through an oxidative polymerization reaction; preparing an electrode; dissolving sulfuric acid in water to prepare a sulfuric acid electrolyte with a concentration of 1 mol / L; and preparing the self-supporting flexible conductive polymer supercapacitor material. According to the invention, a three-dimensional cross-linked conductive polymer micro-nano structure is obtained by utilizing supramolecular doping, and the three-dimensional cross-linked conductive polymer micro-nano structure has good conductivity and specific surface area and has relatively good specific capacity;since PVA and triaminobenzeneboronic acid are added, the flexibility of polyaniline is greatly improved through covalent bonds, and flexibility and self-supporting are achieved; and by adjusting the conductivity and flexible performance, a current collector is not needed in design and preparation, weight is reduced, and the energy density of the whole device is improved.

The invention provides boron-phosphorus co-doped activated carbon for adsorbing aromatic-ring-containing compounds in water and a preparation method of the boron-phosphorus co-doped activated carbon,and belongs to the technical field of treatment of organic pollutants in water. The preparation method comprises the steps of uniformly mixing boric acid, phytic acid, a carbohydrate and water, and sequentially carrying out hydrothermal reaction, drying, calcining, water washing and drying on the obtained product to obtain the boron-phosphorus co-doped activated carbon. According to the preparation method, boric acid and phytic acid are used as pore forming agents together, so that the preparation cost can be reduced, and the advantage of in-situ pore forming is maintained. Boron doping and phosphorus doping are introduced into the boron-phosphorus co-doped activated carbon at the same time, and the electron donating capacity of phosphorus counteracts the reduction of aromatic ring chargedensity caused by boron doping, so that the intensity of the EDA effect is maintained, and the adsorption performance of the activated carbon on aromatic ring-containing compounds in the adsorption application of the aromatic ring-containing compounds in water is improved.

The invention relates to the field of genetic engineering, in particular to a low-temperature neutral phytase PhyH with double structure domains as well as a gene and application thereof. The invention provides the neutral phytase PhyH from Bacillus sp.HJB17, and the amino acid sequence of the neutral phytase PhyH is shown as SEQ ID NO.1; and the invention provides a gene PhyH for coding the neutral phytase. The phytase provided by the invention has good pH stability and still keeps higher enzyme activity in the alkaline range; the phytase has higher relative enzyme activity in a low-temperature range; the phytase has double structure domains, an N terminal is an incomplete BPP (Butyl Pivalate Peroxide) phytase structure domain and a C terminal is a complete phytase structure domain. In short, the PhyH obtained by the invention can be used as the low-temperature neutral phytase which can be applied to cultivation of fish; the PhyH-DI structure domain can increase the catalytic efficiency of other phytases and is good for improving the production and the application of the other phytases.

The invention relates to a method for preparing a special foliar fertilizer for organic agricultural crops. The method comprises the following steps: by taking wheat germ oil residue as a raw material, firstly, decomposing grease in oil residue by using a lipase preparation, and then decomposing protein by using a protease preparation, so as to produce amino acids and plant active substances; filtering, reserving liquid for later use, and decomposing an organic phosphorus compound in a solid part by using a phytase preparation, so as to generate available phosphorus; filtering and reserving liquid for later use; finally, mixing two parts of liquid, so as to prepare the foliar fertilizer with high amino acid content and abundant available phosphorus nutrition. The foliar fertilizer prepared by the method obtains a good yield-increasing effect by a field experiment.

The invention belongs to the electrochemistry and nano material technical fields, and especially relates to a graphene nano material-modified glassy carbon electrode based on phytic acid dispersion, a preparation method and an application of the electrode in detection of acetaminophen. The preparation method is as follows: graphene is dispersed in a phytic acid solution, and is subjected to ultrasonic treatment for 4-8 h to obtain a phytic acid dispersion liquid of graphene; a clean glassy carbon electrode is soaked in the phytic acid dispersion liquid of graphene, subjected to self-assembly for 1-4 h, washed and dried; the mass concentration of the phytic acid solution is 30-60%. The glassy carbon has advantages of electrode low preparation cost, simple preparation method and environment protection. The glassy carbon electrode can be applied to detection of acetaminophen, and has good electrocatalytic oxidation activity to detection of acetaminophen. The glassy carbon electrode is advantaged by fast response time, wild linearity range, good reproducibility high stability and freedom from interference of coexisting electroactive substances.

The invention belongs to the technical field of microbiological engineering modification, and specifically provides a Trichoderma reesei mutant strain for highly producing temperature-resistant phytase and application of the Trichoderma reesei mutant strain. A preservation number of Trichoderma reesei is CCTCC NO:M2018836. The Trichoderma reesei mutant strain is capable of efficient recombinant expression of the phytase, the enzyme activity of the phytase in shake flask fermentation liquid reaches up to 1950U / ml and is increased by 54.5% than that before mutation, and the enzyme activity of the phytase in fermented supernate of a 20L tank reaches up to 16680U / ml and is increased by 49.8% than that of an original strain. The heat resistance of the phytase obtained from recombinant expression of the Trichoderma reesei mutant strain is remarkably increased, and the phytase has very high enzyme activity and can be widely applied to the feed production field.

The present invention relates generally to methods of use and compositions useful to reduce dark spots, age spots, and / or unwanted pigmentation of skin. The composition includes a combination of niacinamide, phytic acid, Rosmarinus officinalis leaf extract, Chondrus crispus extract, and a combination of Saxifraga sarmentosa extract, Carica papaya (papaya) fruit extract, and Psidium guajava (guava) fruit extract. The composition is effective to reduce dark spots, age spots, and / or unwanted pigmentation of skin. The composition is effective to inhibit tyrosinase and / or Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ) activity. The composition is further effective to reduce melanin content in skin, particularly dark skin, in some instances by at least 30%.

The invention, which relates to the technical field of food processing, provides flavored hot pickled mustard tubers and a preparation method thereof. The flavored hot pickled mustard tubers is prepared by using the following raw materials: green vegetable heads, a soaking solution, phytic acid, sodium erythorbate, disodium stannous citrate, citric acid, lactic acid bacteria, sodium alginate, protease and table salt. According to the invention, the flavored hot pickled mustard tubers having crisp and tasty taste, moderate acidity, and unique flavor has extremely low nitrite content and is a nutritional and safe preserved hot pickled mustard tuber product; the sensory quality is improved, the nutritional value is increased, the shelf life is prolonged, so that the healthy consumption concept of modern consumers is met; the lactic acid bacteria are used for pickling the mustard tuber, so that the cost is saved, the food safety of the hot pickled mustard tuber is improved, the flavor of the hot pickled mustard tuber is improved, the quality and functionality of the hot pickled mustard tuber are enhanced. The preparation method is a novel safe and reliable process; production of harmful microorganisms in the preparation process of the hot pickled mustard tuber is reduced, the flavor of the hot pickled mustard tuber is ensured and the shelf life is prolonged.

The invention discloses a novel hydrogel electrode patch for health monitoring and a preparation method thereof, wherein the specific preparation method comprises the steps: dissolving glycerin, gelatin, polyvinyl alcohol and phytic acid in water, converting the mixture into a clear transparent solution in an environment of 70-100 DEG C, pouring the transparent solution into a mold for freezing treatment, and thus obtaining the novel hydrogel electrode patch for health monitoring. The novel hydrogel electrode patch disclosed by the invention is colorless and transparent, has good spatial stability, water locking property, electrical conductivity, antibacterial property, adhesiveness and easy complete stripping property, and has a good application prospect in the aspect of human-computer interaction medical monitoring equipment.

The invention discloses a zinc phytate@zeolite imidazate skeleton-8 / molybdenum disulfide nanocomposite and a preparation method and application thereof, and belongs to the technical field of anti-corrosion coatings. Polydopamine modified molybdenum disulfide is used as a template, and zinc phytate@zeolite imidazate skeleton-8 nanoparticles are grown in situ on the surface of the template. Zinc phytate@zeolite imidazate skeleton-8 is evenly distributed through the lamellar structure of molybdenum disulfide, the smoothness of the composite coating is effectively improved, the corrosion rate of a metal base material is reduced, the zinc phytate@zeolite imidazate skeleton-8 nanoparticles on the surface of molybdenum disulfide can be stimulated by a surrounding corrosive medium to be cracked, and the corrosion inhibitor zinc phytate and 2-methylimidazole are released. A metal anti-corrosion emulsion prepared on the basis is a nano composite material with passive and active protection functions, and the anti-corrosion performance of a polyacrylate coating is synergistically improved.

The invention belongs to the technical field of composite materials, and particularly relates to a phytic acid-vanadium pentoxide composite material, a preparation method thereof, an electrode and a battery. The composite material is a lamellar material, and phytic acid coats the outer surface of vanadium pentoxide and is intercalated between adjacent layers of vanadium pentoxide. The composite material can effectively overcome / improve the problems of relatively poor specific capacity and cycle performance caused by difficult deintercalation of zinc ions and poor structural stability in a cycle use process when the existing vanadium pentoxide is used as an aqueous zinc ion battery positive electrode material.