The invention provides a hot melt adhesive membrane for bonding a bi-metallic composite plate and a preparation method of the hot melt adhesive membrane. The adhesive membrane is prepared by the following steps: adopting one or several from polyacrylate rubber, polyethylene-acrylic acid (ester) copolymer or polyvinyl chloride-vinyl acetate esters copolymer as adhesive-based resin; adopting one or several blends from polyethylene, chloridize polyethylene, polyethylene-octene copolymer, ethylene propylene rubber, polyethylene-vinyl acetate, polymethyl methacrylate, polymethyl methacrylate-butadiene-styrene copolymer, polystyrene, thermoplastic polyurethane and polyvinyl chloride as a second matrix; adding a certain amount of thickening agents, fillers and functional additives after pre-mixing; and then casting to form the membrane. The hot melt adhesive membrane has high bonding strength and lasting stability, excellent weather resistance, aging resistance of salt mist, and water resistance, and is suitable for compounding and bonding of metal materials such as stainless steel, galvanized steel, iron, aluminum, copper, titanium alloys and the like.