The present application belongs to the field of
nutrition and
health food and biological
medicine technology, and particularly relates to a preparation method of walnut
peptide and application of the walnut
peptide in intelligence-improving products. The preparation method comprises the following steps: walnut
protein is re-dissolved in water, stirred uniformly, and then homogenized to obtain an enzymolysis substrate; bromelain and ficin are added to the enzymolysis substrate for enzymolysis, and then the
enzyme is inactivated; a compound
microbial agent is added to the inactivated solution for
fermentation, and then centrifuged after inactivation; the supernatant is collected, ultra-filtered, purified, and then the fraction is collected;
ethanol is recovered, and then dried to obtain the walnut
peptide. The present application significantly improves the walnut
protein hydrolysis efficiency by optimizing the enzymolysis
system and process parameters, and the obtained walnut peptide has strong
antioxidant activity and high purity of
small molecule peptides, can effectively protect
nerve cells and improve memory, and can be used for preparing
antioxidant, memory-improving food, health products or medicines.