The invention relates to a method for preparing an antase titanium dioxide nanowire array by virtue of a one-step process. The method comprises the steps of adding a certain amount of crystal face inducer to a container at first, secondly, adding acetic acid and ultrasonically stirring until dissolving, thirdly, adding N,N-dimethylacetamide and ultrasonically stirring until being even, finally, adding a certain amount of tetrabutyl titanate, evenly mixing and adding the obtained mixture to a hydrothermal reaction in which a conductive substrate subjected to spin-coating treatment previously, heating for reacting, taking out a sample after the reaction is finished, washing the surface of the sample, and drying to obtain the antase titanium dioxide nanowire array. The one-dimensional antase titanium dioxide nanowire array prepared by the method has the characteristics of controllable dimension, simple preparation process, low cost and the like. The one-dimensional antase titanium dioxide nanowire array can be used as a working electrode material for a dye-sensitized solar cell and a quantum dot solar cell.