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46results about How to "Applicable application" patented technology

Method for preparing exosome freeze-dried powder of human amniotic mesenchymal stem cells

The invention relates to the field of stem cells, discloses a method for preparing exosome freeze-dried powder of stem cells, and particularly relates to a method for preparing exosome freeze-dried powder of human amniotic mesenchymal stem cells. The method for preparing the exosome freeze-dried powder of the human amniotic mesenchymal stem cells disclosed by the invention comprises the following steps: mixing trehalose with exosomes of the human amniotic mesenchymal stem cells as a cryoprotectant; filtering and removing bacteria by virtue of a filter membrane; and firstly carrying out ultralow temperature pre-freezing, and then carrying out low-temperature freezing at a certain vacuum degree. The preparation method disclosed by the invention is simple to operate, safe and effective. An experiment proves that compared with a conventional preparation method of the freeze-dried powder, the morphologies and the activity of the exosomes of the human amniotic mesenchymal stem cells can be well kept by the method for preparing the exosome freeze-dried powder of the human amniotic mesenchymal stem cells disclosed by the invention; and the method is suitable for long-term preservation and application of the exosomes of the human amniotic mesenchymal stem cells.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD

Method for extracting cyclic adenosine monophosphate (cAMP) from red jujubes

InactiveCN101525363AThe process consumes less energySimple processSugar derivativesUltrafiltrationFood additiveFiltration membrane
The invention relates to a method for extracting cyclic adenosine monophosphate (cAMP) from red jujubes, which comprises the steps of cleaning of the red jujubes, soaking of the red jujubes at normal temperature, precooking and pulping, zymohydrolysis on pectin mixed enzyme, filter pressing, anion resin combination, formic acid elution, nano-filtration and interception of formic acid with nano-filtration membrane, and purification, thus obtaining a finished product. The method solves the problems of complex current process, long period, low product purity and the like, and has the advantages of rich raw material sources, simple process, large processing amount, high extraction rate, high product purity and the like; the product is a natural non-toxic product; the finished product cAMP can be used for medical raw materials or used as food additive; the extracted byproduct red jujube juice can be continuously and deeply processed to promote the industrial chain of comprehensive development and achieve pollution-free production; the modern nano-filtration technology is applied to industrial production, therefore, not only the cost is reduced, but also a better path for deeply processing the red jujube raw material is provided, and the consumption of energy is greatly reduced; and the method not only has important economic benefit, but also has remarkable social benefits.
Owner:TIANJIN UNIV OF SCI & TECH

Underground interchange bifurcate tunnel underground excavation structure

InactiveCN106894825AIncrease tunnel spacingAchieve separationTunnelsEnvironmental geology
The invention relates to an underground interchange bifurcate tunnel underground excavation structure which comprises at least one grade of transition segment with the section width gradually increased, a large-span tunnel maximum-section segment connected with the last grade of transition segment, a multi-arch tunnel segment, a small-distance tunnel segment and a separated tunnel segment. A reserved middle rock pillar bifurcating a tunnel is arranged between the small-distance tunnel segment and the separated tunnel segment. A middle partition bifurcating the tunnel is arranged in the middle of the multi-arch tunnel segment. One end of the bifurcated small-distance tunnel segment is connected with the multi-arch tunnel segment. One end of the bifurcated multi-arc tunnel segment is connected with the large-span tunnel maximum-section segment. The bifurcated separated tunnel segment is connected with the other end of the small-distance tunnel segment correspondingly. Compared with the prior art, according to the underground interchange bifurcate tunnel underground excavation structure, through conversion and transition of various tunnel section structures, bifurcation of the underground interchange tunnel is achieved, and the underground interchange bifurcate tunnel underground excavation structure is particular suitable for design and application of an urban underground interchange road.
Owner:SHANGHAI MUNICIPAL ENG DESIGN INST GRP

Preparation method of Ni-SiC compound coating on surface of aluminum alloy and electroplating solution thereof

The invention discloses a preparation method of a Ni-SiC compound coating on the surface of an aluminum alloy. The preparation method adopts an anode type surfactant which is hexadecyl trimethyl ammonium bromide for replacing a cathode type surfactant which is lauryl sodium sulfate adopted by the invention at present. The preparation method is characterized by increasing the concentration of an anode activator NiCl2 while reducing current density and the concentration of silicon carbide in a plating solution, obtaining a fine and dense compound coating at an electroplating rate of 1-2 micron per minute, stirring by using air with volume flow being 4-7L/min and fully dispersing the silicon carbide, simultaneously adding CTAB to improve the wettability of the silicon carbide and obtain a fine and dense compound coating with high content of uniformly-dispersed silicon carbide. Through the preparation method of the Ni-SiC compound coating on the surface of the aluminum alloy, the technical problem of low tensile performance and low abrasion resistance of the aluminum alloy can be solved; the Ni-SiC compound coating with high adhesion strength, high content of uniformly-distributed hard silicon carbide particles, high hardness and high abrasion resistance can be prepared on the surface of the aluminum alloy.
Owner:NORTHWESTERN POLYTECHNICAL UNIV

Preparation method for microarray biochip

The invention relates to a preparation method for a microarray biochip. M print spray heads are used to constitute a spray head group, the spray heads are installed in the direction parallel to the X direction, and are arranged along the Y direction; a biochip substrate and the spray head group move step by step relatively along the Y direction, the action of a corresponding nozzle on the spray head corresponding to each step position is controlled by a driving signal, and a biomolecule solution of one index is sprayed onto the preset position of one or a plurality of microarray biochip substrates arranged along the X direction; and through the sequential action of the M spray heads, the biomolecule solutions of M indexes are sequentially sprayed onto the preset position of the biochip substrate to form a sample application array. The preparation for the microarray biochip used for multi-index and multi-user parallel detection can be realized. Compared with the existing product, the biochip prepared in the method disclosed by the invention has the characteristics of high speed, high precision, wide applicability, high efficiency, small cross contamination, easiness in maintenance and use, low manufacturing cost and the like, and the method is very suitable for the large-scale production of microarray biochips and is also suitable for scientific research and application.
Owner:UNIV OF SHANGHAI FOR SCI & TECH

Preparation method of edible water balls by performing synthesizing on pectin extracted from tamarind seeds

The invention discloses a preparation method of edible water balls by performing synthesizing on pectin extracted from tamarind seeds. The preparation method of the edible water balls by performing synthesizing on the pectin extracted from the tamarind seeds comprises the following steps: performing pretreatment on tamarind seeds; preparing tamarind seed gum powder; performing hydrolyzing, decoloring and precipitating on the tamarind seed gum powder; and preparing the edible water balls. A gelation technology is effectively used during the preparation process of the edible water balls. The edible water balls are synthetized by using sodium alginate, the tamarind gum and the like as raw materials, wherein the added tamarind gum extracted from the tamarind seeds has high hydrophilicity; so that, very well protecting and stabilizing effects can be achieved during the preparation processes of the edible water balls. Thus, the prepared edible water balls are not liable to break, and stablerin performance; moreover, membrane of the prepared edible water balls is tougher, more translucent, brighter and easier to degrade, so that the current environment protection theme are better met. The preparation method of the edible water balls by performing synthesizing on the pectin extracted from the tamarind seeds effectively solves the environment protection problem of existence of plasticparticles in plastic bottles, so that the preparation method of the edible water balls is suitable for industrial and large-scale production; and thus, the preparation method of the edible water ballsis wide in application range, as well as suitable for large-scale promotion and application.
Owner:FOSHAN UNIVERSITY

Method for efficiently separating and purifying caffeoylquinic acid isomers from mulberry leaves

The invention relates to the field of separation purification of natural products, and provides a method for efficiently separating and purifying caffeoylquinic acid isomers from mulberry leaves. Themethod comprises the following steps: performing ultrasonic wave enhanced extraction on the mulberry leaves, and performing separation purification by adopting an original pH induction liquid-liquid extraction technology, a macroporous adsorption resin enrichment technology and a high-speed countercurrent chromatography rapid separation technology to obtain the three caffeoylquinic acid isomers with purity of 93% or more. According to the method provided by the invention, caffeoylquinic acid rapidly enters an organic phase by the pH induction liquid-liquid extraction to reduce extraction times, so that the time is saved, chemical reagent consumption is reduced, and the industrial costs are reduced; and the method realizes the purpose of separating the caffeoylquinic acid isomers from the mulberry leaves by using the high-speed countercurrent chromatography rapid separation technology for the first time, the method has the advantages of simple steps, low solvent consumption, a short separation period, high product purity and a high yield, and is suitable for industrial production and application.
Owner:BEIJING FORESTRY UNIVERSITY +1

Single system micro-service splitting method based on domain event driving

The invention relates to a single system micro-service splitting method based on domain event driving. The method is technically characterized by comprising: constructing a domain event model, and generating a multi-dimensional interface relevancy comprehensive matrix; identifying candidate micro-services from the multi-dimensional interface relevancy comprehensive matrix; according to the candidate micro-service, constructing a micro-service interface calling relation chain of each layer, and performing decoupling processing to obtain a micro-service result; and evolving each micro-servitization result from a three-layer architecture mode to a domain-driven design layered architecture mode. The concept of field-driven design is combined, the method does not depend on specific software components, needed relevant information is directly mined from single application, a single system is described into a field event graph model, in the process, and the method more pays attention to the essence of micro-service splitting instead of a certain specific software technology. Therefore, the splitting method is basically not restricted by a specific development technology, and can be well suitable for most of application programs.
Owner:TIANJIN UNIV

Flat band bonding machine

The invention discloses a flat band bonding machine. The flat band bonding machine comprises a rack, a control system, a supporting frame, a lower pressing strip, heating sheets, a temperature sensor, a fixed plate, an air cylinder, an upper pressing strip and an iron sheet, wherein the control system is arranged in the rack; the supporting frame is arranged on one side of the top of the rack; a groove is formed in the middle part of the supporting frame; the lower pressing strip is longitudinally fixed on the groove; a plurality of heating sheets are arranged on the lower pressing strip; the temperature sensor is additionally arranged on one side of the lower pressing strip; the fixed plate is arranged above the supporting frame; the air cylinder is arranged on the fixed plate; the upper pressing strip is arranged below a telescopic rod of the air cylinder; the upper strip is located between the fixed plate and the lower pressing strip; the iron sheet is arranged below the upper pressing strip; the temperature sensor, the air cylinder and the heating sheets are respectively connected with the control system. The flat band bonding machine disclosed by the invention has the advantages that the production efficiency is high, the operation is simple, the blank of polyurethane flat band production equipment in the market is filled, the bonding firmness is high, the market demands are well satisfied and the flat band bonding machine is suitable for being widely popularized and used.
Owner:苏州震昇机电设备有限公司
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