The invention discloses an anti-microbial TPU material and a preparing method thereof. The anti-microbial TPU material comprises raw materials including, by weight, 1-15 parts of polyether polyol, 15-55 parts of isocyanate, 3-25 parts of a chain extender, 10-35 parts of polyamide, 0.5-6 parts of an anti-microbial agent, 5-40 parts of polyethylene glycol, 0.1-5 parts of a mildew preventive, 0.05-4 parts of an antioxidant, 1-15 parts of organic bismuth, 0.1-8 parts of an antiblocking agent and 0.1-20 parts of a filler. The above raw materials are weighed according to the parts by weight and are evenly mixed; and an obtained mixture is dried, and extrusion is carried out in a casting machine to obtain the anti-microbial TPU material. The anti-microbial TPU material has the technical advantages of high abrasive resistance, good elastic toughness, high hydrolytic resistance, high hardness, high yellowing resistance, high transparency, high tearing strength and the like; the anti-microbial agent, the mildew preventive, the antioxidant and the antiblocking agent are added, and therefore the anti-microbial TPU material has the beneficial effect of being high in anti-microbial and mildew-preventing performance, and wide application of the TPU material in the field with high anti-microbial requirements is promoted; and the anti-microbial TPU material and the preparing method have the wide market prospect.