The invention provides a waterborne high-speed ink-jet printing heat transfer ink for a Single-pass Kyocera nozzle. The waterborne high-speed ink-jet printing heat transfer ink is prepared from the following raw materials of nanometer color paste, polyhydric alcohols, a wetting agent, a penetration quick-drying agent, an emulsifier B, a sterilant and deionized water, wherein the color paste is prepared from the following raw materials of salt-free refined superfine dispersive dye color powder, a dispersant, an emulsifier A, glycerin, a sterilant, a defoamer and deionized water. The waterborneKyocera high-speed ink-jet printing heat transfer ink has the advantages that the waterborne high-speed ink-jet printing heat transfer ink is suitable for Single-pass high-speed ink-jetting printing of Kyocera KJ4B-600DPI nozzle, the printing is smooth, the standby property is good, the quick-drying property is good, the printing property is stable, and the wire drawing is avoided; the color stability and reduction property are good, the register and color are conveniently matched in the printing process, the printing quality is high, the consistency of the finished product is good, the comprehensive cost is low, the waste of dye ink is avoided, and the resource is saved; the physical and chemical properties are excellent, namely that the level of water washing property is 4 to 5, the level of dry and wet scratching property is 4 to 5, and the sun-resistant property is 3.5 to 5; the environment-friendly effect is realized, and the poison and harm are avoided; in the printing process, the platemaking is not needed, the wastewater and waste gas are not produced, and the high-efficiency and environment-friendly effects are realized.