The invention discloses a method for determining a lime mixing amount ratio in expansive soil, relates to the technical field of civil engineering, and aims at solving the problems that in the relatedart, the expansive soil is improved through the lime, and after lime metering exceeds a certain value, the mechanical index and the water stability of the lime stabilized soil are reduced, and the method comprises the following steps that S1, taking the expansive soil and the lime; S2, preparing an EDTA standard titer line according to the water content controlled by construction; S3, taking 3-6%of lime, and uniformly mixing with the lime to obtain a mixed soil sample; S4, culturing bacillus megaterium liquid and preparing cementing liquid; S5, adding diluted 60-80% bacillus megaterium liquid into the mixed soil sample, uniformly stirring, and standing for 2 hours; S6, after 2 hours, sucking out the bacillus megatherium liquid, adding 60-80% of cementing liquid, continuing to solidify, uniformly stirring, and standing for 22 hours; and S7, sucking out the bacillus megaterium liquid and the cementing liquid, and repeating the steps S4 and S5 seven times.