The invention discloses a traditional Chinese medicine composition of medicinal liquor for resisting fatigue and improving sexual function and a preparation method of the traditional Chinese medicine composition, belongs to health-care nourishing medicinal liquor, and particularly relates to health-care nourishing liquor prepared by taking cistanche extraction concentrate as a main functional raw material. Raw materials for preparing active ingredients comprise cistanche, prepared rhizome of rehmannia, acanthopanax, Chinese chive seeds, pyrolusite, schisandra chinensis, fructus chebulae, fructus psoraleae, nandina, diaphragma juglandis, herba epimedii, clove, radix aconiti lateralis praeparata, rhizoma cimicifugae, dried ginger, pepper, ginseng, dogwood, astragalus membranaceus, rhizoma polygonati, lucid ganoderma, gastrodia elata, Chinese yam, poria cocos, tricholoma matsutake, fructus lycii, mulberry, fructus alpiniae oxyphyllae, pericarpium citri reticulatae, cordyceps militaris, maca powder, lalang grass rhizome, liquorice, rock candy and pure grain white spirit. The invention has the advantages of accurate control of prepared active components, reasonable and scientific raw material compatibility, simple preparation of finished wine, small color deviation of the finished product, and convenience for large-scale production. The product has the health-care functions of resisting fatigue and improving the sexual function.