The invention belongs to the technical field of non-ferrous metal analysis and particularly relates to an electropolishing preparation method of a sample for EBSD (Electron Back-Scattered Diffraction) analysis of a titanium-aluminum alloy. The electropolishing preparation method comprises the following steps: firstly, carrying out earlier stage treatment on the sample: determining the size of the sample to be smaller than 7mm*6mm*4mm, and carrying out standard metallographic treatment and mechanical polishing on the testing surface; secondly, setting up a portable device: connecting the sample with a positive pole of a power supply, connecting a stainless steel plate with a negative pole of the power supply and matching the bottom end of electrolyte with a magnetic stirrer; thirdly, preparing the electrolyte: selecting 4 to 12 percent of perchloric acid, 55 to 65 percent of methyl alcohol and the balance of n-butyl alcohol according to the volume ratio, wherein the total amount of the electrolyte is 700ml; putting the electrolyte into a beaker with the volume of 1L; fourthly, carrying out electrolysis treatment, washing and drying: controlling the temperature to be 30 DEG C below zero to 20 DEG C below zero, controlling the voltage to be 25 to 35V and controlling the polishing time to be 40 to 55 seconds. The electropolishing preparation method disclosed by the invention has the advantages of large surface area for treating the sample and suitability for preparing titanium-aluminum alloy samples with various components and various test purposes, which are prepared by different processing method. In addition, the electropolishing preparation method has the advantages of easiness and flexibility in operation, no restriction from site facilities and the like, good polishing effect and high rate of finished products; the device has the characteristics of high mobility, low cost, easiness in promotion and wide application prospect.