The invention relates to a compound neomycin sulfate composition for treating pet abscess and a preparation method of the compound neomycin sulfate composition. The composition is prepared by uniformly mixing the following components by weight: 1-3 parts of neomycin sulfate, 3-5 parts of erythromycin thiocyanate, 1-2 parts of trimethoprim lactate, 1-3 parts of cetrimonium bromide, 3-5 parts of piperitol, 50-70 parts of liquid paraffin, 7-9 parts of glycerol, 0.4 part of ethylparaben, 1-3 parts of essence, 80-113 parts of distilled water and the like. The compound neomycin sulfate composition has the beneficial effects that the formula is reasonable, preparation is simple, curative effects are very remarkable, and furthermore, clinical application show that the compound neomycin sulfate composition has special effect for treating pet abscess.