The application discloses an
embryo in-vitro culture and in-situ
monitoring system and method, and belongs to the field of online monitoring of
embryo in-vitro culture. The
system comprises: a high-precision
microscope, which is a table type fixed structure and is provided with a stage; a culture unit, which is based on the structural design of the stage and is arranged on the stage, is used for accommodating embryos and providing a
culture environment required by in-vitro development of the embryos; a
thermostat, which seals the related part of the
microscope containing the stage and provides a stable temperature environment for the culture unit; and a
control unit, which is connected with the culture unit, is used for providing a multi-channel
perfusion culture pipeline and driving and can perform time-periodic
perfusion culture. The application wraps the
microscope by the
thermostat and adopts
cascade closed-loop
temperature control to provide a stable temperature and
humidity environment; realizes fine
shear force stimulation through time-periodic
perfusion; realizes in-situ high-resolution imaging through
adaptation of the high-precision microscope, does not need to transfer embryos, and significantly improves
imaging quality and experimental efficiency.