12 results about "Ion regulation" patented technology

The invention provides a breeding method suitable for breeding litopenaeus vannamei in saline-alkali soil, and relates to the technical field of aquaculture. According to the breeding method suitable for breeding the litopenaeus vannamei in the saline-alkali soil, the proportion of Ca < 2 + > to Mg < 2 + > to K < + > in a water body is optimized to be 2.8: 1: 0.3 by customizing a compound ion regulation and control solution, SO4 < 2-> / Cl <-> is controlled to be 0.14-0.8, and the pH is stabilized to be 8.2-8.8 in combination with a citric acid-sodium citrate buffer system; a slow-medium-fast segmented domestication strategy is adopted to adapt to different development stages of the fries; ethanol clostridium protein (CAP) is added into the feed to be combined with compound probiotics, so that immune metabolism regulation and control are enhanced. Experiments prove that according to the method, the survival rate of the litopenaeus vannamei seedlings reaches 86.3% + / -2.1%, the culture period is shortened by 12-15 days, the hepatopancreas SOD activity is improved by 42.6%, the vibrio inhibition rate reaches 78.5%, and the method is remarkably superior to the prior art. The method does not need large-scale pond transformation, adapts to different types of saline-alkali soil water bodies, realizes efficient green culture, and has important application value.

According to the ion regulation and control device, the ion guide device, the control method, the device, the equipment and the ion guide system provided by the invention, the first guide element and the deflection force field generation module are arranged, under the condition that ions are injected into the first guide element, the first guide element is controlled to apply the reverse electric field opposite to the ion transmission direction, and the deflection force field is generated; thereby trapping ions within the first guide element; controlling the deflection force field generation module to work so as to enable the ions in the first guide element to generate radial deflection; and under the condition that offset is completed, the first guide element is controlled to cancel the reverse electric field so that the first guide element can move in the transmission direction, ions in the first guide element can generate radial offset through the deflection force field generation module, the ions can be more uniformly distributed in the radial direction, and therefore the space area utilization rate is increased, and the transmission efficiency is improved. And the influence of the space charge effect is reduced.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses a nitrate ion transport channel gene PbrSLAH3b and application of the nitrate ion transport channel gene PbrSLAH3b in promoting growth of pear pollen tubules. The method comprises the following steps: extracting total RNA (Ribonucleic Acid) of Dangshan pears from pollen of Dangshan pears; carrying out reverse transcription on the RNA to obtain cDNA (complementary deoxyribonucleic acid); and by taking the cDNA as a template, carrying out amplification to obtain the pear pollen tube nitrate transport channel gene PbrSLAH3b, the PbrSLAH3b gene provided by the invention belongs to a slow anion channel SLAC / SLAH family member, the PbrSLAH3b is remarkably up-regulated in expression in the early growth stage of a pollen tube, and the PbrSLAH3b is positioned in a plasma membrane. The invention verifies that PbrSLAH3b can form a functional anion channel with NO3 <-> selectivity under the condition of not depending on proton gradient or phosphorylation state, and the PbrSLAH3b has relatively strong selectivity on NO3 <->. Therefore, the PbrSLAH3b gene can mediate the absorption and transport of the pollen tube to NO3 <-> and maintain the polarity growth directivity of the pollen tube, and has important application value for revealing the ion regulation mechanism of the growth of the fruit tree pollen tube and improving the reproductive development of the fruit tree.

The present application relates to the field of tobacco production, and particularly relates to a tobacco leaf chlorine ion content regulation method, comprising the following steps: 1) tobacco field chlorine ion detection: detecting the chlorine ion content in tobacco field soil and tobacco field irrigation water respectively; 2) classifying the tobacco field according to the chlorine ion content of the tobacco field obtained in step 1); 3) controlling chlorine in the tobacco field according to the classification obtained in step 2). The present application realizes effective control of the chlorine ion in the tobacco field by adopting a hierarchical regulation strategy in the process of large-scale agricultural production, with relatively low cost and simpler means. The tobacco leaf chlorine ion regulation method designed in the present application fully coordinates the aspects of tillage, cultivation, field management and irrigation in large-scale agricultural production, and strengthens the chlorine ion regulation strategy from the perspective of material circulation, and has stable effect and practicability.

The application discloses a PIP-seq technology-based adjustable osmotic pressure single-cell sequencing kit and a detection method, and through a wrapping buffer with adjustable osmotic pressure and a non-toxic digestion system, high-throughput sequencing of marine high-osmotic living cells and freshwater low-osmotic living cells without chemical fixation is realized for the first time, and the retention rate of sensitive cell groups (such as stinging cells and freshwater ion-regulating cells) is doubled. By only changing the buffer formula, marine and freshwater samples can be seamlessly switched without changing the core reaction process, and the kit has strong versatility, and successfully solves the osmotic pressure barrier problem that has long plagued the field of aquatic biology.

The invention discloses a sex hormone binding globulin calibrator diluent and an application thereof. The calibrator diluent comprises the following components and contents: 50-100mM of a buffer solution; 0.01% to 0.1% of estradiol or an estradiol derivative; 0.5%-2% of a protein protective agent; 1%-10% of an anti-freezing agent; 0.1-1% of metal ions; 0.1%-0.5% of a preservative; 0.1%-2% of an ion regulator; 1%-10% of a saccharide protective agent; 0.1%-1% of a complex; and the balance of water. The in-vitro diagnostic reagent calibrator is prepared in a liquid form, and compared with freeze-dried calibrators adopted by well-known foreign manufacturers such as Rogowski and other domestic and overseas manufacturers in the market, the in-vitro diagnostic reagent calibrator has the advantages of convenience and rapidness in use; the problem of inaccurate calibration product concentration caused by operation errors possibly introduced when doctors in clinical laboratories in hospitals redissolve the calibration product is avoided, and under the synergistic effect of the components, the unstable dimer structure of the SHBG can not be degraded in a diluent for a long time, so that the ability of the calibration product to correct a reagent curve is effectively ensured.

The invention provides an intelligent electrodialysis demineralization method and system for improving the thermal stability of goat milk. According to the method, mineral ions in goat milk are selectively removed through an electrodialysis technology, an intelligent closed-loop control system based on real-time conductivity monitoring is adopted, the ion removal proportion is accurately regulated and controlled, and particularly the molar concentration ratio of sodium ions to potassium ions is controlled to be (1.2-1.4): 1. The thermal stability of the treated goat milk is remarkably improved, the thermal stability time at 140 DEG C can be prolonged by 80-150%, and the precipitation rate after UHT treatment is lower than 2.0%. The intelligent control electrodialysis technology is applied to precise ion regulation and control of the goat milk for the first time, the technical problem that the goat milk is poor in high-temperature processing stability is solved, and the method has important industrial application value.

The present application relates to the technical field of aquatic pathogenic bacteria culture, and particularly relates to a preparation method of a freshwater vibrio high-efficiency culture composite medium containing a nucleotide compound.A freshwater vibrio high-efficiency culture composite medium containing a nucleotide compound, in terms of weight parts, includes the following functional components: 2000-5000 parts of a carbon source, 1500-3000 parts of a nitrogen source, 225-405 parts of a precursor substance, 65-125 parts of an amino acid, 55-110 parts of an energy system, 650-1300 parts of an ion regulator, 35-85 parts of a membrane stabilizer, and 10-20 parts of an antioxidant.The present application solves the technical problem that the prior art lacks a culture method that can effectively promote the growth of freshwater vibrio and ensure the motility activity of the freshwater vibrio.Through reasonable formulation and process design, the present application breaks through the bottleneck of the existing freshwater vibrio culture technology, provides support for the research, targeted prevention and control, and efficient detection method establishment of aquatic pathogenic bacteria, and has a significant application prospect.

This invention discloses an integrated evaporation material and its preparation method for high-concentration brine treatment and power generation based on ion regulation, as well as a water treatment and energy harvesting system, belonging to the technical fields of solar interface evaporation. The material uses polyurethane sponge as a porous framework, constructs a photothermal and conductive network by impregnating conductive graphite with a sodium alginate suspension, and then crosslinks it twice with sodium alginate and calcium chloride, with polystyrene sulfonic acid impregnation introduced in between to form an ion-regulated gel layer containing sulfonate groups, constituting a three-layer composite structure. The material has a simple preparation process and low cost, and under one day of sunlight, the evaporation rate in a 20wt% sodium chloride solution is ≥3.6 kg·m³. ‑2 ·h ‑1 Furthermore, it is salt-free and can generate an open-circuit voltage of ≥800 mV and a short-circuit current of ≥600μA. It has a salt ion rejection rate of >99.9%, enabling efficient desalination and stable power generation of high-concentration brine, making it suitable for large-scale production applications.

The invention relates to the technical field of culture of aquatic pathogenic bacteria, in particular to a preparation method of a compound culture medium for efficiently culturing freshwater vibrio containing a nucleotide compound. The invention relates to a compound culture medium for efficiently culturing freshwater vibrio containing a nucleotide compound, which comprises the following functional components in parts by weight: 2000-5000 parts of a carbon source, 1500-3000 parts of a nitrogen source, 225-405 parts of a precursor substance, 65-125 parts of amino acid, 55-110 parts of an energy system, 650-1300 parts of an ion regulator, 35-85 parts of a membrane stabilizer and 10-20 parts of an antioxidant. The scheme is used for solving the technical problem that a culture method capable of effectively promoting the growth of freshwater vibrio and ensuring the movement activity of the freshwater vibrio is lacked in the prior art. Through reasonable formula and process design, the bottleneck of an existing freshwater vibrio culture technology is broken through, support is provided for research, targeted prevention and control and establishment of an efficient detection method of aquatic pathogenic bacteria, and the method has remarkable application prospects.

The invention belongs to the field of mineralized materials for water purification, and discloses slow-release mineralized porous composite particles as well as a preparation method and application thereof. Comprising the following components in parts by weight: 10-40 parts of calcined dolomite, 5-20 parts of tourmaline, 30-70 parts of zeolite and 5-20 parts of a fluxing agent. The preparation method comprises the following steps: mixing calcined dolomite serving as a basic material with tourmaline and zeolite, tabletting, and calcining and forming by taking sodium carbonate as a fluxing agent to obtain porous composite particles with mesopores as main pores and macropores as auxiliary pores. The porous composite particle has a stable mineral element slow release rate, can meet the requirements of drinking water mineralization treatment and drinking water taste improvement, has an excellent ion regulation and control capability, can effectively prevent excessive dissolution of heavy metal elements, harmful elements and hardness ions, is high in safety, does not have a scaling risk, and is suitable for popularization and application. The device is suitable for household direct drinking water, water outlet of a water purifier and other scenes. The raw material source is wide, the preparation process is simple, convenient and controllable, and the application prospect is wide.

The invention discloses biomimetic mineralization dental cement with an antibacterial-mineralization synergistic function as well as a preparation method and application of the biomimetic mineralization dental cement. The biomimetic mineralized dental cement is composed of a solid phase system and a liquid phase system, wherein the solid phase system comprises calcium phosphate-based inorganic particles and an antibacterial polysaccharide functional layer which is constructed in situ on the surfaces of the calcium phosphate-based inorganic particles through hydrogen bonds and electrostatic interaction; the liquid phase system is an ion regulation solution containing complexing components. The antibacterial polysaccharide participates in induction of mineral phase growth in the curing process through a pre-compounding process to form a biomineralization-like composite structure, so that the antibacterial and mineralization functions are synchronously realized. The material is in the shape of injectable paste, the curing time can be adjusted within 5-240 min, the compressive strength after curing reaches 5-30 MPa, and the material has good X-ray radiation resistance, biocompatibility and broad-spectrum antibacterial performance. The biomimetic mineralized dental cement is suitable for the fields of dental root canal filling, tooth remineralization repair, periodontal tissue repair and the like.