The invention relates to a feed additive for improving the production performance of lactating sows, a preparation method and application thereof, and belongs to the technical field of sow feeding. The above-mentioned feed additives include enteromorpha, pyrroloquinoline quinone, kelp, motherwort, buliuxing, malt, brown sugar, licorice, purslane, Bupleurum and compound amino acids. Its preparation method includes: mixing kelp, motherwort, blancia, malt, brown sugar, licorice, purslane and Bupleurum, decocting, concentrating, and mixing with enteromorpha, pyrroloquinoline quinone and compound amino acids. The preparation method is simple, low in cost and good in controllability. The prepared feed additive can effectively improve the immune level of lactating sows and promote lactation; and its raw materials are all green additives, which are safe and environment-friendly. Applying it to the feed of lactating sows can pass benefits through the mother and offspring, improve the health status of suckling piglets, improve the growth performance of piglets and the economic benefits of breeding.