The invention discloses biomass three-dimensional porous graphene and a preparation method thereof. The preparation method comprises the steps: uniformly mixing biomass powder with inorganic salt, performing high-temperature cracking reaction under the protection of inert gas, cooling to room temperature, washing an inorganic salt template with hot water of 90-100 DEG C, filtering, washing to recover molten salt, drying an obtained black solid product, and thus obtaining the biomass three-dimensional graphene. The three-dimensional porous graphene is prepared from the biomass material and thewater-soluble inorganic salt, and biomass cracking is catalyzed by the molten salt, so that the method has the characteristic of short high-temperature cracking time; in the cooling stage, nano or micron salt particles formed by recrystallization of molten salt are used as templates and pore-forming agents for carbon precipitation embedded into the molten salt, high-specific-surface-area three-dimensional graphene is precipitated on the surfaces of salt particles, the preparation process is simple and easy to implement, the preparation period of the three-dimensional graphene can be shortened,and the method is more environmentally friendly compared with a method for preparing graphene through an oxidation-reduction method.