The invention discloses a root growth promoting agent for tobacco, its preparation and application. The root growth promoting agent comprises the following ingredients: 100-150 weight parts of potassium nitrate, 50-100 weight parts of urea, 10-50 weight parts of potassium dihydrogen phosphate, 10-50 weight parts of amino acid powder, 5-10 weight parts of zinc sulfate, 5-10 weight parts of ferrous sulphate, 5-10 weight parts of boric acid, 5-10 weight parts of sodium alginate, 5-10 weight parts of glucose, 5-10 weight parts of compound sodium nitrophenolate, and 5-10 weight parts of paclobutrazol. The root growth promoting agent disclosed herein can simultaneously provide tobacco with organic and inorganic nutrition, macro and trace nutrients, and root growth promoting factors with physiological activity, has the effect of improving tobacco rhizosphere environment, and can rapidly induce the rooting and growth of seedlings. The root growth promoting agent disclosed herein can be applied by blade spraying, hole irrigation, root irrigation and the like. Compared with the control group, the total number of roots which is subject to the application of the root growth promoting agent increases by 5.04-35.19%, the root dry weight increases by 5.69-32.47%, and the root volume increases by 7.99-36.75%. The root growth promoting agent disclosed herein can be widely applied in tobacco production.