The invention discloses a denitration catalyst regeneration solution and a preparation method thereof as well as a catalyst regeneration method. The denitration catalyst regeneration solution comprises the following raw material ingredients in parts by weight: 4-6 parts of vanadyl oxalate, 0.08-0.10 part of cerium nitrate, 0.11-0.13 part of lanthanum nitrate, 0.05-0.07 part of copper nitrate, 0.4-0.6 part of silica sol ST-O and 95 parts of deionized water. The catalyst regeneration method comprises the following steps of firstly, evenly mixing vanadyl oxalate and the deionized water; then, feeding cerium nitrate, lanthanum nitrate and copper nitrate into mixed solution, and fully stirring until the mixture is completely dissolved; finally, feeding silica sol ST-O, and continuing to stir until homogeneous phase is obtained; and sequentially carrying out strong scavenging and ultrasonic cleaning on an inactivated denitration catalyst module, soaking the module in cleaning solution for 18-25 minutes, continuing soaking the module in the regeneration solution for 25-40 minutes, taking out the denitration catalyst module and drying. After the denitration catalyst regeneration solution and the catalyst regeneration method are adopted, the activity of the inactivated denitration catalyst can be efficiently recovered, and the temperature range of the catalyst activity is widened.