The invention discloses UV-curing light guide adhesive and a preparation method thereof; the UV-curing light guide adhesive comprises a photoinitiator, a monomer, polybutadiene dimethacrylate, aliphatic polyurethane acrylate, a rheological agent, a leveling agent, a dispersant, a coupling agent, a defoaming agent, and light guide powder; the preparation method comprises the steps of first, mixing well a photo-curing monomer, polybutadiene dimethacrylate and a coupling agent; second, adding aliphatic polyurethane acrylate, a leveling agent, a dispersant, a defoaming agent and a photo-curing agent, and mixing well; third, adding light guide powder, and mixing well; fourth, adding a rheological agent, and mixing well to obtain a finished product; the prepared UV-curing light guide adhesive has excellent advantages of excellent yellowing resistance, peel strength, impact resistance, high luminance, uniformity and the like; the UV-curing light guide adhesive is applied to the surface of reflecting film by means of printing, roll coating, brush coating, and curtaining and is cured by UV irradiation, the technique omits the procedure of manufacturing a light guide plate, production efficiency is improved, and production cost is lowered.