The invention discloses a preparation method of 
tuna protein peptide. The method comprises the following steps of 1, firstly, selecting fresh 
tuna skin as raw materials, then, mixing the 
tuna skin andwater according to the ratio of 1:30, then, heating the mixture in a closed container to reach the 
boiling point, keeping the 
water temperature to 100 DEG C all the time, and in the process, obtaining a 
mixed solution after 2-3 h; 2, cooling the obtained 
mixed solution to 40-65 DEG C, then, adding an 
enzyme into the 
mixed solution, performing standing for 1-2 h, and then performing sufficient stirring; 3, the preparation method of the tuna 
protein peptide is high in yield, good in 
antibacterial activity and good in beautifying and 
skin moisturizing effect; meanwhile, the adopted raw materialscontain no 
hazard substances, no environment 
contamination is caused, the materials are wide in source, the preparation technology is simple, the product has the effects of maintaining beauty, keeping young, promoting 
cell growth and resisting aging, and the great important effect is brought into play for vital activity.