The invention relates to high-purity rice bran protein and rice bran fibers, and further relates to a preparation method of the high-purity rice bran protein and the rice bran fibers. The preparation method includes the steps of raw material pretreatment, first size mixing and enzyme reaction, second size mixing and enzyme reaction, separate washing, drying and the like. According to the method, through superfine grinding and amylase and cellulose hydrolyzing, components of defatted rice bran are effectively separated, a whirlcone is utilized to conduct the washing treatment, and therefore separate washing efficiency is greatly improved. Compared with spray drying, flash drying equipment is utilized, investment is saved by about 40%, operating cost is saved by about 35%, in rice bran protein products, the protein content is over 80%, the ash content is below 3%, the fat content is below 0.5%, the protein extraction ratio is over 75%, and in rice bran fiber products, the fiber content is over 70%, the ash content is below 4%, and the fat content is below 1%. The high-purity rice bran protein and the rice bran fibers all can be widely applied to the field of health food, nutritious food and the like, and additional value of rice deep processing is greatly improved.