The invention relates to a method for preparing monodisperse mesoporous bioactive glass microspheres through a template method, belonging to the field of biomedical materials. A silicon source, a phosphorus source, a calcium source, an iron source, an alkali metal or alkaline-earth metal source is adopted as a raw material, a triblock copolymer or alkyl quaternary ammonium salt type cation is taken as a surfactant, and an in-situ sol-gel method is performed in a three-dimensional ordered macroporous polymer or a carbon template to synthesize the ordered mesoporous bioactive glass microspheres. The bioactive glass microspheres prepared according to the invention have the characteristics of good biocompatibility, biodegradability, ordered mesoporous structure, higher specific surface area, magnetism, monodispersibility of particles, environmental stability and the like; and in comparison with various medicament delivery carrier materials which are extensively researched currently, the bioactive glass microspheres have more comprehensive advantages and further have broad application prospects as novel medicament controlled release carrier materials.