31 results about "Camellia sinensis (Plant)" patented technology

The invention belongs to the technical field of gene engineering, and discloses a camellia sinensis(L.)O.Kuntze) WRKY29 gene and an application thereof to improving cold resistance of plants. The nucleotide sequence of the camellia sinensis(L.)O.Kuntze) WRKY29 gene is shown as SEQ ID NO.1 in a sequence table. The amino acid sequence of the protein coded by the camellia sinensis(L.)O.Kuntze) WRKY29 gene is shown as SEQ ID NO.2 in the sequence table. The expression mode of the camellia sinensis(L.)O.Kuntze) WRKY29 is related to the response of camellia sinensis(L.)O.Kuntze) to low-temperature stress, the gene is over-expressed to transform plants, the cold resistance of the plants can be improved, the expression of CsWRKY29 is inhibited through an antisense oligonucleotide technology, and the damage of camellia sinensis(L.)O.Kuntze) leaves at low temperature is obviously aggravated. The cloning of the gene not only is beneficial to exploring the effect of a WRKY transcription factor in the cold resistance process of the camellia sinensis(L.)O.Kuntze), but also is beneficial to promoting the genetic improvement process aiming at enhancing the cold resistance of the camellia sinensis(L.)O.Kuntze) and promoting the sustainable development of the tea industry, so that the gene has a great application value.

The invention discloses a novel pesticide, and a preparation method and an application thereof, and belongs to the technical field of preparation of pesticides. The novel pesticide comprises, by weight, leaves of Salix babylonica.L, Piper methysticum Forst, Camellia Sinensis leaf, Anabasis aphylla L., paulownia leaf, Derris fordii Oliv., garlic, neem, horseradish, Pterocarya stenoptera leaves, Cortex Fraxini, Sophora flavescens, Euphorbia soongarica, pyrethrum, onion, patrinia herb, purslane, Radix Scutellariae, aloe, Cortex Magnoliae Officinalis and water, and the novel pesticide is prepared through the steps of medicine solution extraction, concentration, drying, crushing, sieving and disinfection. The novel pesticide has the advantages of nontoxic component, no residue, no nuisance, simple production method, low cost and good application effect, can effectively prevent and treat yellow spiders, Parlatoria pergandii Comstock, papilios, leaf miner and other plant pests, has a primary killing rate reaching 96.06% or above, has no medicine resistance, and can be used for 4-6 years.

The invention belongs to the technical field of cosmetics, and particularly relates to a plant-derived composition for preventing hair loss and / or growing hair. The composition is prepared from the following components: a saccharomycete lysate extract, inulin, alpha-glucan oligosaccharide, glycine, a LARIX EUROPAEA wood extract, a CAMELLIA SINENSIS leaf extract and a Mentha Piperita extract. The composition is suitable for alopecia, treatment and hair growth caused by different reasons.

The invention provides a traditional Chinese medicine composition for dredging microcirculation. The traditional Chinese medicine composition is mainly prepared from sea buckthorns, a saussurea involucrate culture, phytosterol, hawthorns, zaocys dhumnades, earthworm protein, acerola cherry fruits, eucommia ulmoides male flowers, cordyceps militaris, cyclocarya paliurus leaves, Ningxia medlar, ginkgo, kudzuvine roots, purslane, fragrant solomonseal rhizomes, cassia seeds, gynura procumbens, camellia sinensis flowers, tuckahoe, pawpaw, angelica dahurica roots, lotus seeds, solomonseal rhizomes,corn stigma, curcumae longa, taurine, nicotinic acid and yeast beta-glucan. The traditional Chinese medicine composition provided by the invention is prepared by taking pure natural plants as raw materials to perform accurate compounding according to traditional Chinese medical theory (meridian circulation and microcirculation) by adopting the principles of ''monarch, minister, assistant and guide'' and ''mutual generation and constraining'' of traditional Chinese medicines, and performing warm extraction and purification. Through modern biological polymerizations, the general microcirculationis quickly dredged, blood is purified, fat is dissolved, thrombus is discharged, blood vessels are softened, blood fat and blood pressure are reduced, cardiovascular and cerebrovascular diseases areimproved, metabolism is promoted, a sub-health state is improved, various visceral organs are comprehensively conditioned, unbalanced organs in a body are balanced, and health is restored.

The present invention makes it possible to efficiently produce an N-methyl transferase with 7-methylxanthine N3 methyl transferase, theobromine N1 methyl transferase, and paraxanthine N3 methyl transferase activities that can be utilized as an industrial, food, or medical enzyme. In addition, the present invention makes it possible to modify the caffeine biosynthesis metabolism of caffeine producing plants, plant tissues, or plant cells, thereby efficiently producing caffeine metabolism based compounds or modifying the production of caffeine in the plants.

Disclosed is a process for the enzymatic preparation of a gamma-glutamyl compound. The process comprises a step of contacting a gamma-glutamyl donor and a gamma-glutamyl acceptor with an aqueous medium comprising a gamma glutamyl transpeptidase enzyme. The enzyme is derived from a plant belonging to the Graminaceae or Leguminaceae family, or from Camellia sinensis.

The present invention relates to Production of transgenic tea (Camellia sinensis (L.) O. Kuntze) through biolistic

The invention discloses a crossbreeding method for a new variety of tea trees. The crossbreeding method comprises the following steps: 1) selecting parents; 2) collecting pollens; 3) conducting artificial pollination; 4) picking seeds; 5) breeding seeds; 6) conducting field planting; and 7) screening excellent single plants. The invention belongs to the field of crossbreeding of tea trees and particularly provides an artificial crossbreeding method by taking Keemun camellia sinensis var.sinensis cv.Zhuye tea as a female parent and camellia sinensis var.assamica cv.Fengqing-Daye tea as a male parent. Through hybridization, single plant selection, asexual propagation, strain comparison and regional test, a new variety of tea trees is bred. The variety inherits excellent botanical characteristics of the female parent, cold resistance of the tea trees is improved, and the crossbreeding method for a new variety of tea trees enables tea trees to have strong tenderness, obvious three-dimensional germination, strong growth vigor, strong stress resistance and strong fecundity.

The invention relates to a camellia sinensis planting method. According to the method, arbores that grow slowly are planted at the edge of a camellia sinensis planting area, the arbores are 2 to 3 meters away from camellia sinensis at the outmost periphery, a one-row and one-plant planting mode or a two-row and two-plant planting mode is adopted, and organic fertilizers such as decomposed manure of poultries and livestock as well as retting straws are utilized. The beneficial effects are that a problem that biocenoses singly rely on camellia sinensis is solved, rich biological diversity is provided, and large-scale insect pests are less likely to break out; pesticides and fertilizers are prevented from being excessively used during plantation of camellia sinensis; and produced tea is rich in selenium, popular in markets, and high in output value.

The invention discloses an ecological camellia sinensis planting method. The ecological camellia sinensis planting method comprises the following steps of (1) selecting planting fields: selecting sloping fields of which the elevation is 600-800 meters and the slope is 10-20 degrees, and red land of which the weight content of soil organic matters is 2% or above, of which the pH is 4.5-6.5 and of which the depth of soil layers is 50cm or above, wherein the average annual rainfall is 1500mm or above; (2) planting melia azedarach on the planting fields according to the row spacing being 15-20 meters and the plant spacing being 10-15 meters, and when melia azedarach plants reach to 3 meters or above, beginning to planting the camellia sinensis; (3) planting the camellia sinensis: longitudinally furrowing and ridging on the sloping fields, wherein the width of ridges is 40-60cm, the width of furrows is 20-30cm, the depth of the furrows is 20-30cm, planting the camellia sinensis on the ridges, and planting rosemary in the furrows; and (4) performing daily management: applying 1500-2000 kilograms of farmyard manure per mu every year. According to the ecological camellia sinensis planting method disclosed by the invention, under the situation that the growth of the camellia sinensis is not influenced, diseases and insect pests of the camellia sinensis can be prevented and treated, and besides, the economical added value of land can be increased.

The present invention explored PLGA NPs synthesis using plant extracts as surfactants by nanoprecipitation process. PLGA NPs synthesized using Camellia sinensis, Dendrocalamus hamiltonii, Ficus palmata and Rubus ellipticus leaf extracts were further explored for encapsulation, controlled and sustained release of well-known antioxidant molecule, curcumin. This plant extract based nanoprecipitation process for the synthesis of PLGA NPs can be further explored for encapsulation of many other antioxidant molecules of therapeutic importance.

A weight loss product comprises a mixture of Alchemilla vulgaris, Olea europaea, Cuminum cyminum, and Mentha longiflora (in the weight proportion 12:10:5:4), to which additional ingredients have been added to increase weight loss efficacy. The additional ingredients may include, but are not limited to, Gamma-oryzanol, Caffeine, Cirsimarin, Fucoxanthin, Guggulsterones, Evodiamine, Forskolin, Sclareolide, Chromium, Hydroxycitric acid, Pinolenic acid, Conjugated linoleic acid, Potato protein, Raspberry ketone, Capsaicin, Synephrine, Dehydroepiandrosterone, Phenylethylamine, Orlistat, Glucomannan, or Vitamin C, or sources thereof, as well as herbal ingredients such as Guarana, Yerba Mate, Damiana, Green tea, Green coffee bean, Cinnamon, White kidney bean, Garcinia Cambogia, Nopal Cactus, Hoodia, Yohimbine, Malpighia glabra, Eurycoma Longifolia, Caralluma Fimbriata, Citrus aurantium, Gymnema Sylvestre, Lyceum barbarurn, Aloe Vera, Cayenne, Pomegranate, Blueberry, Billberry, or extracts of such herbal ingredients, and plant sources of antioxidants. A preferred additional ingredient is caffeine provided by caffeine anhydrous, Coffea Arabica, Coffea canephora, Camellia sinensis, Ilex paraguariensis, Guarana, Theobroma cacao, and kola nut. Other additional ingredients may be used as well in accordance with specific disclosed formulations.

The invention discloses a yellow camellia sinensis planting method. The yellow camellia sinensis planting method comprises the following steps: (1) soil preparation is carried out, concretely fertilesandy soil is selected as planting soil, then 50-100 kg of lime powder is scattered into the inner part of each mu of deep pits for carrying out disinfection, the pH value of the soil is adjusted to be 5-6, and then deep pits with a depth of 60-80 cm and a width of 50-60 cm are dug every 1-1.5 m on the surface of the soil; and (2) fertilizing is carried out, concretely 1000-1200 kg of farmyard manure is added into each mu of land subjected to finishing in the step (1), and then 30-35 kg of a compound fertilizer is added. According to the yellow camellia sinensis planting method, soil treatmentneeds to be carried out on the selected land in advance in a camellia sinensis planting process, the soil condition can be more beneficial to the growth of the camellia sinensis by changing the pH value of the soil, the growth of the camellia sinensis can reach an optimal state by changing the fertilizing and watering in the planting process, and the strength of the soil around the camellia sinensis can be reinforced through the addition of planted corn seedlings, so that the camellia sinensis cannot be toppled over due to rain wash.

The invention discloses application of a hexose transporter gene CsSWEET1a of camellia sinensis in improving low temperature resistance of plants. Biological function verification by overexpression ofthe gene in arabidopsis proves that the CsSWEET1a gene provides a gene resource for breeding of new varieties of low-temperature-resistant plants by increasing the content of sucrose and glucose in plants and improving the low temperature resistance of the arabidopsis, and has better potential application value.

Antioxidant cosmetic skin care compositions formulated to combat conditions associated with free radical damage and oxidative stress are provided. The compositions contain effective amounts of one of several active agents, including an agent derived from one or more of the plant species: Lycium barbarum, Punica granatum, Vitis vinifera, Aspalathus linearis, and Camellia Sinensis as well as cosmetically acceptable carriers. These cosmetic compositions find use in improving the appearance of aged or damaged skin.

The invention discloses sapindus mukurossi cleansing foam and a preparation method thereof. The sapindus mukurossi cleansing foam is prepared by taking out, mixing and dissolving the following components in parts by weight of a component 1 including 48-51 parts of water, a component 2 including 9-11 parts of PEG-60 corn glycerides, a component 3 including 8-10 parts of sapindus mukurossi mixtures,a component 4 including 7- 9 parts of decyl glucoside, a component 5 including 4- 6 parts of PEG-8 caprylic / capric glycerides, a component 6 including 3-5 parts of hydrolyzed collagen mixtures, a component 7 including 3-5 parts of gleditsia sinensis seed extraction mixtures, a component 8 including 2-4 parts of camellia sinensis leaf extract mixtures, a component 9 including 1-3 parts of hamamelis virginiana extract mixtures, a component 10 including 1-3 parts of lophatherum gracile extract mixtures, a component 11 including 1-3 parts of aloe barbadensis leaf mixed extracts, a component 12 including 0.2-0.4 part of polyaminopropyl biguanide, a component 13 including 0.04-0.06 part of parfum, and a component 14 including 0.08-0.11 part of PEG-10 hydrogenated castor oil. The pure plant extracts are adopted as a conditioner, the skin can be deeply nourished, and all the medicinal agents are kept in a reasonable range, so that effective conditioning can be guaranteed, and strong side effects cannot be generated.

The invention discloses an anti-hair loss composition, an anti-hair loss washing care product and a preparation method thereof, and relates to the field of cosmetics. The product comprises the following raw materials: a fruit extract of phyllanthus emblica, a leaf extract of tea (camellia sinensis), a stachyurus himalaicus extract, a larix europaea extract and a limonia acidissima extract. The plant extracts provide different anti-hair-loss mechanisms, have mutual synergistic interaction, activate the activity of hair follicle cells and promote the cells to secrete growth factors, are low in toxicity to the cells and have excellent anti-hair-loss and hair-growing effects.

The invention discloses a camellia-plant-primary-solution extracting device. A rectangular frame is arranged inside a constant temperature box, a steel wire net plate is fixedly arranged inside the rectangular frame, the top of the constant temperature box is communicated with one end of a U-shaped pipe, a multi-joint stretching pipe is arranged at the other end of the U-shaped pipe, the top-end outer wall of the multi-joint stretching pipe is fixedly welded to the inner wall of the end of the U-shaped pipe, the multi-joint stretching pipe penetrates through a columnar through hole on a piston, the bottom-end outer wall of the multi-joint stretching pipe is fixedly welded to the inner wall of the columnar through hole, the piston is located inside a column cylinder, and the outer wall of the piston is in compact contact with the inner wall of the column cylinder. According to the camellia-plant-primary-solution extracting device, the piston is arranged inside the column cylinder, the piston inconveniently enters, camellia-plant-primary-solution steam flowing out of the multi-joint stretching pipe is compressed, and therefore the liquidation speed of the camellia-plant-primary-solution steam is increased; meanwhile, the piston can scrape a liquefied camellia-plant primary solution located on the inner wall of the column cylinder, the liquefied camellia-plant primary solution falls into the storage box through a conical cylinder, and therefore the collection efficiency of the camellia-plant primary solution is improved.

The invention discloses a method for grafting large branches of Camellia japonica, comprising the following steps: (1) selection of stock; (2) selection of scion; (3) treatment of stock; (4) treatment of scion; (5) grafting: scion embedded in stock In the "V" groove, the two sides are closely connected. Tie the joint tightly with thin iron wire, and make the excess part face down, then wrap the joint with film, and wrap the scion with film at the same time. The plastic film does not Completely wrap the excess iron wire so that the interface will not accumulate water and at the same time keep it breathable; (6) Manage after grafting. The invention improves the grafting survival rate of camellia camellia, promotes the growth and flowering of scions, shortens the cultivation cycle of large-scale plants, and has a good role in promoting the development of camellia camellia industry.

The invention discloses a molecular marker site linked with epicatechin (epicatechin, EC) content of camellia sinensis trees and an application of the molecular marker site. For the first time, the inventor of the invention finds that an SNP molecular marker site pertinent to the epicatechin content of the camellia sinensis trees on a camellia sinensis tree genome Scaffold2233: 468642, and the genotype is significantly correlated with the epicatechin content. The relevance and significance verification result indicates that compared with GG genotype samples and GA genotype samples, the epicatechin content in tea soup dry substances corresponding to AA genotype samples has significant differences. Judgment is performed through statistics, when the genotype is double mutation AA, the epicatechin content in dry substances in the camellia sinensis trees is higher than that of the normal average level obtained from wild type GG or single mutation genotype GA by large probability. The detection method for detecting the site is further established, can be used for evaluating the epicatechin content of the camellia sinensis trees to be further used for screening and molecular breeding of high-EC (epicatechin) camellia sinensis tree resources, and has great research value.

The invention discloses a gene for adjusting and controlling development of epidermal hair of camellia sinensis and quality formation of tea leaves and an application. The nucleotide sequence of a CsMYB184 gene is as shown in SEQID NO.1 in a sequence table, and the amino acid sequence of protein coded by the CsMYB184 gene is as shown in SEQID NO.2 in the sequence table. The expression pattern of the CsMYB184 is highly correlated to distribution of tea fine long hair; when the gene is used for overexpressing and transforming plants, the development defect phenotype of the epidermal hair of transgenic plants can completely return to normal; and through an antisense oligonucleotide technique, the expression of the CsMYB184 is restrained, the content of characteristic compounds in camellia sinensis leaves can be notably reduced. Cloning of the gene not only facilitates understanding a molecular mechanism for establishing the epidermal hair shape of the camellia sinensis, but also facilitates recognizing a molecular mechanism for forming important appearance and quality agronomic character namely tea fine long hair, a theory basis is provided for quality breeding of camellia sinensis, and the breeding process of specific camellia sinensis varieties is accelerated. The gene disclosed by the invention has great application value.

The invention relates to a soothing and antibacterial infant hair washing and bathing bubble and a preparation method thereof. The bubbles is prepared from water, 9%-11% of decyl glucoside, 9%-11% ofcocamidopropyl betaine, 1%-5% of glycerol, 5%-10% of sodium lauroamphoacetate, 1%-5% of panthenol, 1%-5% of PEG-7 olive oil sodium carboxylate, PEG-75 shea butter glyceride, tocopheryl acetate, calendula officinalis extract, aloe vera extract, centella asiatica extract, polygonum cuspidatum root extract, scutellaria baicalensis root extract, camellia sinensis extract, glycyrrhiza glabra root extract, chamomile recutita extract, sodium benzoate, potassium sorbate and citric acid. The soothing and antibacterial infant hair and bath bubbles is mild and anti-bacteria, one bottle is used for multiple purposes, clean and soothing are achieved, the bubbles contain plant extracts, the skin problem is solved while cleaning, and the effects of eczema inhibition, antisepsis and anti-inflammation areachieved.

The invention relates to the field of Camellia sinensis (L.) O. Ktze. planting, specifically to a processing method capable of increasing the yield of Camellia sinensis (L.) O. Ktze.. The method specifically includes the steps: 1), pruning Camellia sinensis (L.) O. Ktze. in the germination period of Camellia sinensis (L.) O. Ktze. in spring, wherein the pruning method comprises the specific steps:(1) bottom pruning: removing all branches and bud leaves within the height range of 25 cm from the ground to the trunk; and (2) tip pruning: pruning top branches of Camellia sinensis (L.) O. Ktze., performing cutting from the positions with tree forks and outward-growth branches, and performing pruning in the direction of the branches in a flat and clinging mode; 2), one month after pruning of Camellia sinensis (L.) O. Ktze., spraying a chemical reagent to Camellia sinensis (L.) O. Ktze. when complete healing of wounds of the branches; and 3) burying compound fertilizer in the root of Camellia sinensis (L.) O. Ktze. after spraying of the chemical reagent, wherein before the compound fertilizer is buried, a circular groove with a width of 5-10 cm and a depth of 20-30 cm is dug in the horizontal ground at a position which is 20-30 cm away from the root when the root of Camellia sinensis (L.) O. Ktze. is adopted as a center, then the compound fertilizer is buried in the groove, and the compound fertilizer comprises cow dung, dry leaves of Camellia sinensis (L.) O. Ktze., potassium dihydrogen phosphate, coated urea and phosphorus.

The invention discloses a camellia sinensis pest repellant from plant-source extract products. The camellia sinensis pest repellant comprises (by molar mass): 1 part of carvacrol, 0.90-1.10 parts of capsaicin, 0.45-0.55 part of gastrodin and 0.30-0.37 part of naringenin. The camellia sinensis pest repellant is good for plant-source pest repelling, is efficient, low-toxic and easily-degraded, and causes no residue or drug resistance.