The invention discloses a moisturizing and repairing essence lotion and a preparation method thereof. The moisturizing and repairing essence lotion comprises the following raw materials of microzyme fermentation lysate filtrate, a grape seed extract, a gentian root extract, a plant bionic sebum agent, polyglutamic acid, an emulsifying agent, potassium cetyl phosphate, an emollient, polydimethylsiloxane, butyrospermum parkii sheabutter oil, cetearyl alcohol, tocopheryl, pentaerythrityl tetra-di-t-butyl hydroxyhydrocinnamate, a chelating agent, allantoin, hydroxyethyl urea, glyceryl polymethacrylate, panthenol, xanthan gum and the like. The moisturizing and repairing essence lotion provided by the invention has the advantages of being notable in use efficacy, mild in properties, non-irritating and free from side effects, and is suitable for all of people to use; all the raw materials are reasonably compounded, so that the obtained moisturizing and repairing essence lotion is uniform andsoft in texture, enables face skin to be tender, fine and smooth, flat, glossy and transparent; and a production technology provided by the invention is simple, low in cost, safe and environmentally-friendly and has extremely high economic value.