The invention relates to the technical field of pharmaceutical analysis, and discloses a method for determining the
zinc content in a
calcium zinc gluconate preparation. The determination method comprises the following steps: reacting a
calcium zinc gluconate preparation with a complexing agent to obtain a
zinc ion-complex; determining the
absorbance of the
zinc ion-complex by using an
ultraviolet-visible spectrophotometric method; and calculating the zinc content based on the
standard curve. According to the determination method provided by the invention, the complexation reaction and the
ultraviolet spectrophotometry are combined, the zinc content in the
calcium zinc gluconate preparation is calculated by the
standard curve, the result accuracy is high, and the defect that the determination result is inaccurate due to the reason that an end point is subjectively judged by virtue of color and the like in an EDTA
titration method is overcome; the complexing agent [o-[2-(2-hydroxy-5-sulfo-phenylazo) benzal] hydrazinobenzoic acid and
xylenol orange are only complexed with zinc ions under a specific pH condition, so that the specificity is high, and the interference of other raw and auxiliary materials in the calcium zinc gluconate preparation can be avoided.