The invention discloses a method for preparing a three-dimensional spherical anatase type TiO2 photo-anode, relating to a solar cell photo-anode. The method comprises the steps of: preparing a reaction water solution, wherein the reaction water solution contains 0.1-1M sodium hydroxide and 5wt%-30wt% of hydrogen peroxide; then, cleaning a titanium sheet, placing the cleaned titanium sheet in the reaction water solution, sealing the reaction water solution in a hydrothermal kettle for reaction, and then cooling to obtain light yellow precipitates; then, washing and filtering the light yellow precipitates, drying and sintering the light yellow precipitates to obtain three-dimensional TiO2 nanospheres; and mixing and stirring the three-dimensional TiO2 nanospheres, polyethylene glycol, deonized water and absolute ethyl alcohol to prepare slurry, cleaning and drying FTO electrocondcutive glass, coating a slurry layer on the FTO electroconductive glass, sintering and then carrying out TiCl4 treatment to obtain the three-dimensional spherical anatase type TiO2 photo-anode. The method is simple to operate, short in operation time, low in cost, remarkable in improving the performance of a dye-sensitized solar cell, and easy for industrialization.