The invention provides a method for synthesizing a cobra
antibacterial peptide NA30-CATH through multi-copy integration and high-density
fermentation and application of the cobra
antibacterial peptide NA30-CATH, and belongs to the technical field of
genetic engineering and bioengineering. According to the invention, a series-connection two-copy
plasmid is constructed,
pichia pastoris is taken as a host, two-copy series-connection
protein is successfully expressed, and the expression yield of the cobra
antibacterial peptide NA30-CATH is increased by more than two times. According to the present invention, the cobra antibacterial
peptide NA30-CATH can be synthesized at a high level by using the cobra antibacterial
peptide NA30-CATH and constructing and optimizing the two-copy integration method and optimizing the
fermentation process, and the method has advantages of strong versatility, high expression efficiency,
biological activity, low cost, easy large-scale production, and the like. In addition, the invention further provides application of the cobra antibacterial
peptide NA30-CATH as an
antibacterial agent in the process of breeding the largemouth bass, and the
survival rate of the largemouth bass is increased.