The present invention provides lyophilized levofloxacin composition powder for injection, and relates to the technical field of drugs and drug production. The lyophilized levofloxacin composition powder comprises the following raw material components, by weight: 1.75-2.14 parts of esomeprazole sodium, 0.87-1.07 parts of chitosan nanoparticles, and 94.80-97.38 parts of water for injection. The lyophilized levofloxacin composition powder has the following advantages that: 1) the poor stability problem of the principal agent is overcome, and long-term storage is easily achieved; 2) the composition has an in vivo synergistic effect, can be provided for clinically treating stomach ulcers, gastroesophageal reflux diseases, duodenal ulcers and the like, and has a characteristic of rapid efficacy, and in vitro experiment results verify that the 40 mg of esomeprazole sodium containing the chitosan nanoparticles provides the same gastric acid secretion inhibition effect as the 60 mg of chitosan nanoparticle-free esomeprazole sodium; 3) the patient treatment period is shortened with the enhanced activity, such that the possibility of adverse reaction occurrence caused by esomeprazole sodium is reduced; and 4) the chitosan nanoparticles can be adopted to replace mannitol so as to be adopted as the lyophilized skeleton agent of the lyophilized powder injection, such that the activity effect of mannitol on human body is eliminated.