The invention provides an optimization control apparatus for denitrification carbon source addition in the AO process. The apparatus comprises an A pool, an O pool, a first pipeline, a second pipeline, a third pipeline, an agitator, a water inlet flowmeter, a water inlet COD analyzer, a water inlet ammonia nitrogen analyzer, a dissolved oxygen instrument, a first nitric acid nitrogen sensor and determinator, a second nitric acid nitrogen sensor and determinator, a frequency conversion internal reflux pump, a central control system, a carbon source addition flowmeter, a frequency conversion carbon source addition pump and a carbon source box. The problems of poor denitrification stability, low efficiency and feedback delay existing in a traditional biological A/O denitrification technologyare solved, and the problems existing at present that the control precision is insufficient, the carbon source waste is easily caused, the production cost is increased, and the water output which is stably up to the standard is difficult to be ensured are also solved.