The invention relates to a preparation method and application of litchi seed extract for preventing and treating prostate cancer, and belongs to the technical field of traditional Chinese medicine extracts. The present invention adopts dipping, ultrasonic, microwave, reflux extraction, extraction and other processes to fully destroy the cell wall, accelerate and increase the dissolution of active ingredients, and obtain the litchi core extract of the present invention. The extraction method has high efficiency and good quality of the extract, and can be directly Made into tablets, granules, powders, dripping pills, teas, decoctions, capsules, mixtures or syrups, etc., and added to related foods, beverages or medicines as functional additives for adjuvant therapy or treatment of prostate cancer, Thereby expanding the medicinal scope and value of litchi core.