The invention relates to an agent for promoting fruit coloration of Nanguo pear. The agent is aqueous and dedicated for Nanguo pear, and can increase the 'red fruit' rate by regulating and controlling the synthesis and metabolism of anthocyanidin in Nanguo pear. The agent contains the following components: uniconazole 0.1g / L, sucrose 1g / L, potassium sulfate 1g / L, calcium chloride 3g / L, levulinic acid 0.1g / L, glutamic acid 1.0g / L, tyrosine 0.3g / L, phenylalanine 0.3g / L, boric acid 0.3g / L, Tween 100 1ml / L, and water in balance. The agent is prepared by the following steps: (1) respectively weighting uniconazole, sucrose, potassium sulfate, calcium chloride, levulinic acid, glutamic acid, tyrosine, phenylalanine, boric acid and Tween 100 at the given ratio; (2) respectively dissolving the materials in water, wherein uniconazole is dissolved with methanol; glutamic acid, tyrosine and phenylalanine are dissolves with distilled water at 100 DEG C; and potassium sulfate and calcium chloride are dissolved with distilled water at 50-70 DEG C; mixing after dilution; adding water until the desired concentration; and stirring thoroughly to obtain the final product. The agent can increase the 'red fruit' rate of Nanguo pear by 14%, and is convenient to use and harmless and innoxious to human body and environment.