The invention relates to a marine polysaccharide multilayer composite packaging film with an antibacterial function and a preparation method thereof, belonging to the fields of high polymer materials and food package. The marine polysaccharide multiplayer composite packaging film is prepared by forming a three-layer polyelectrolyte composite film by utilizing the static function of polycation electrolyte chitosan and polyanion electrolyte sodium alginate, and adding natural germicide, i.e. clove oil, to a middle film layer. The preparation method of the packaging film is simple; and the obtained packaging film has favorable mechanical performance, is superior to a low-density polyethylene film, can meet common requirements of food package, has favorable antibacterial controlled-release performance, can effectively prolong the shelf life of foods and can be used for fresh-keeping packaging of various foods, such as meat, cut fruits and vegetables, and the like.