The invention provides an array substrate and a preparation method thereof and a display panel, the array substrate comprises an electrode layer, a gate insulation layer and an active layer, the electrode layer comprises a first metal layer, a second metal layer and a third metal layer which are stacked, a first end of the active layer is electrically connected with the first metal layer, and a second end of the active layer is electrically connected with the third metal layer. The second end of the active layer is electrically connected with the third metal layer, the second metal layer comprises at least two stacked metal sub-layers in the direction from the first metal layer to the third metal layer, and every two adjacent metal sub-layers are arranged in an insulated mode; according to the invention, the first metal layer, the second metal layer and the third metal layer which form the electrode layer are stacked, the channel of the active layer is arranged on the side wall of the electrode layer, the second metal layer is divided into a plurality of metal sub-layers which are stacked, and the thickness and the number of the metal sub-layers can be regulated and controlled, so that the thickness and the number of the metal sub-layers can be regulated and controlled. Therefore, the width-to-length ratio of the channel of the active layer can be regulated and controlled, and the purpose of flexibly regulating the characteristics of the thin film transistor is achieved.