The invention discloses a preparation method of a silver composite antibacterial material of a carbon nano tube. The method comprises the following steps: 1, plasma activating of a carbon nano tube, namely soaking the carbon nano tube in absolute ethyl alcohol, cleaning and drying, and performing plasma activating in a plasma activating instrument; 2, sensitization of the carbon nano tube, namely preparing sensitizing liquid, sensitizing the carbon nano tube subjected to plasma activating in the sensitizing liquid, and drying the carbon nano tube after reaction; and 3, silver plating of the carbon nano tube, namely performing silver plating on the surface of the sensitized carbon nano tube under the protection of inert gases by adopting a vacuum plating machine to obtain the silver composite antibacterial material. According to the preparation method disclosed by the invention, the carbon nano tube is adopted as a supporting carrier, and the carbon nano tube is subjected to plasma activating and sensitizing in sequence, so that the activity of surface functional groups of the activated carbon nano tube is improved, and the silver plating is finally performed, so that agglomeration of silver nanoparticles can be eliminated, the problem of inactivation caused by mutual agglomeration of the silver nanoparticles can be solved, and the antimicrobial activity of the silver nanoparticles can be improved.