The invention discloses a preparation method of a lemon and yellow peach jam, and the jam thereof. The preparation method of the lemon and yellow peach jam comprises the following steps of: weighing yellow peach pulp, lemon juice, high fructose syrup, white granulated sugar, a stabilizer, water, tartrazine pigment, potassium sorbate and yellow peach essence; dividing the yellow peach pulp into twosets according to the weight ratio of 3:2, wherein the part, which is 3/5 of the total weight of the yellow peach pulp, is the first set, juicing the first set of yellow peach pulp to obtain yellow peach syrup, and mixing with the lemon juice, the white granulated sugar and the high fructose syrup to obtain a mixture A; mixing the stabilizer, the tartrazine pigment, the potassium sorbate, the yellow peach essence and the mixture A to obtain a mixture B; putting the second set of yellow peach pulp in soybean protein membrane solution to immerse, taking out, and performing air drying; then, cutting into pieces, putting the cut yellow peaches in new soybean protein membrane solution to immerse again, taking out, and performing air drying to obtain a mixture C; and, putting the mixture C intothe mixture B, stirring, and filling to obtain the finished product. The preparation method in the invention has the advantage of preventing oxidation of yellow peach grains during processing.